大鼠肝脏撞击伤动物模型的制备及评价

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1、大鼠肝脏撞击伤动物模型的制备及评价作者：陆明，汤礼军，田伏洲，麻晓林【摘要】目的建立一种新型大鼠肝脏钝性撞击伤模型，并进行评价。方法健康SD大鼠42只，随机分为3组(n=14)。通过增加了T型二次打击头的改良型生物撞击机，用轻、中、重3种质量的钢球分别撞击1组大鼠，复制出3组损伤程度不同的大鼠肝脏钝性撞击伤模型，即轻度致伤组、中度致伤组、重度致伤组。各组再按观察指标不同，随机平分为A组和B组。测定大鼠A组致伤前后生命体征变化及自然存活数，B组致伤前后血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)含量及致伤后腹腔内出血量。所有大鼠死亡后均进行损伤分级并进行评分。结果

2、各组平均股动脉压致伤后均低于致伤前(P<0.01)，且致伤后平均股动脉压随损伤程度的增加而降低(P<0.01或P<0.05);各组AST、ALT含量致伤后均明显高于致伤前(P<0.01或P<0.05)，致伤后各组AST、ALT含量均随损伤程度的增加而增高(P<0.01或P<0.05);各组腹腔出血量随损伤程度的增加而增多(P<0.01或P<0.05);各组肝脏损伤评分随损伤程度的增加而增高(P<0.01)。结论各组大鼠肝脏损伤程度随钢球质量的增加而加重，分级明显。该大鼠肝脏创伤模型建模过程简单，具有稳定的肝

3、脏损伤的伤情特点,可重复性好,损伤程度均一并可分级调节，费效比低，是一种比较理想的动物实验模型。12【关键词】肝脏损伤;撞击伤;动物模型　　Abstract:ObjectiveToestablishandevaluateanewtypemodelofbluntimpactinjuryofliverinrats.MethodsTotally42healthySpragueDawleyratswererandomlydividedinto3groups(mildinjurygroup,moderateinjurygroupandsevereinjurygroup).B

4、ytheimprovedbiologicalimpactmachinewithaTtypesecondhithead,3groupsofhepaticbluntimpactinjurymodelwereestablishedrespectivelywiththreedifferentweightsteelballs.TheneachgroupwasrandomlydividedintoAgroupandBgrouponaccountofthedifferenceofitemsmeasured.Thedifferenceinvitalsignsbeforean

5、dafterinjuryandthenumberofnaturalsurvivalanimalsafterinjuryinAgroupweremeasured.AST,ALTcontentinserumbeforeandafterinjuryandtheamountofintraabdominalhemorrhageofanimalsafterinjuryinBgroupweremeasured.Theinjuryofallratswasclassifiedandgivenascoreafterthedeath.ResultsTheaveragefemoralar

6、terypressureafterinjuryineachgroupwassignificantlylowerthanthatbeforeinjury(P<0.01),anddecreasedasthedegreeofinjuryincreased(P<0.01orP<0.05).TheserumlevelsofAST,ALTconcentrationafterinjuryineachgroupweresignificantlyhigherthanthosebefore12injury(P<0.01orP<0.05),andincrea

7、sedwithdegreeofinjury(P<0.01orP<0.05).Theamountofintraabdominalhemorrhageineachgroupsignificantlyincreasedwithdegreeofinjury(P<0.01orP<0.05).Thescoreforthehepaticinjurysignificantlyincreasedwithdegreeofinjury(P<0.01).ConclusionThedegreeofliverdamageineachgroupobviousl