塞来昔布对大鼠重症急性胰腺炎血清tnfα，il1β，il6及il8水平的影响

《塞来昔布对大鼠重症急性胰腺炎血清tnfα，il1β，il6及il8水平的影响》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、塞来昔布对大鼠重症急性胰腺炎血清TNFα，IL1β，IL6及IL8水平的影响作者：薛翠华，高振军，李秋萍，许亚平，周春锁，姚俊，付白清【摘要】目的：探讨重症急性胰腺炎(severeacutepancreatitis,SAP)大鼠血清促炎性细胞因子TNFα，IL1β，IL6和IL8的变化及意义，以及应用塞来昔布(celecoxib)的干预效应。方法：胆胰管内注射5%牛磺胆酸钠造模。将动物随机分成对照组、SAP组及塞来昔布组，各组分别于术后3,6,12和24h4个时间点处死大鼠,测血清淀粉酶，TNFα，IL

2、1β，IL6和IL8水平，每次每组5只；取各组术后24h处死大鼠的胰腺组织，行HE染色,分别观察大鼠胰腺组织病理学变化。结果：对照组胰腺组织基本正常,SAP模型组胰腺腺泡结构严重破坏消失，塞来昔布组病理改变明显减轻；SAP组、塞来昔布组大鼠血清中TNFα，IL1β，IL6和IL8水平随病程进展而升高(P<0.01)，各时相SAP组水平明显高于对照组(P<0.01)，塞来昔布组水平明显低于SAP模型组(P<0.01)。结论：TNFα，IL1β，IL6和IL8与SAP的进展有关，塞来昔

3、布可以减轻SAP的炎症反应，抑制促炎性细胞因子的产生。【关键词】重症急性胰腺炎；促炎性细胞因子；塞来昔布10　　［Abstract］Objective:Toexplorethechangesandsignificanceofserumtumornecrosisfactorα(TNFα),interleukin1β(IL1β),IL6andIL8inratswithsevereacutepancreatitis(SAP),andtodetecttheeffectofcelecoxibonthechangesof

4、theseserumcytokines.Methods：TheSAPmodelwasestablishedbyusingretrogradeinjectionofSodiumTaurocholateintocholangiopancreaticduct.Ratsineachgroupwerekilled3,6,12and24h(5ratsforeachtimepoint)afteroperationtodetermineserumlevelsofamylase,TNFα,IL1β,IL6andIL8.Thepan

5、creastissuesoftherats24hafteroperationwereobtainedtoexaminehistopathologicalchangesbyHEdyeing.ThechangesofserumTNFα,IL1β,IL6andIL8indifferentgroupsanddifferenttimepointsweredetectedbyELISAassay.Amylaseinserumwasdetectedbyautomatedbiochemistryanalysis.Results：

6、Thepathologyofcontrolgroupwasrelativelynormal.ThepathologicaldamageinratsofSAPgroupwasveryseverely,butcelecoxibcouldamelioratethepathologicaldamageinratsofSAPgroup.ThelevelsofserumTNFα,IL1β,IL6andIL8inratsofSAPgroupweresignificantlyhigherthanthoseofcontrolgro

7、up(P<0.01)ateverytimepoint.ComparedtoSAPgroup,thelevelsofTNFα,IL1β,IL6andIL8weresignificantlydecreasedin10celecoxibgroup(P<0.01).Conclusion：ThecytokinesofTNFα,IL1β,IL6andIL8wereinvolvedinthepathologicalinjuryofSAP.Celecoxibcanamelioratethepathologic

8、aldamageandinhibittheproductionofproinflammatorycytokineinseruminratsofSAP.　　［Keywords］severeacutepancreatitis；proinflammatorycytokine；celecoxib重症急性胰腺炎(severea