七总皂甙对低氧高二氧化碳性肺血管收缩的影响

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1、七总皂甙对低氧高二氧化碳性肺血管收缩的影响【摘要】目的:探讨三七总皂甙（PNS）对肺动脉平滑肌的舒张作用及其与肺动脉内皮细胞的关系。方法:采用大鼠离体肺主动脉环灌流模型。分别在常氧及低氧高二氧化碳条件下，观察PNS对氯化钾（KCl）及去甲肾上腺素（NE）预收缩内皮完整血管和去内皮血管的舒张作用。结果:①在常氧及低氧高二氧化碳条件下，累积浓度的PNS对60mmol/LKCl及10-5mol/LNE预收缩的血管环均产生剂量依赖性舒张作用(P＜0.05)。②低氧高二氧化碳增强了PNS的肺血管舒张作用，与相应浓度各常氧组相比，均有统计学意义(P＜0.05或P＜0.01)。

2、③常氧条件下，去内皮组与内皮存在组相比，PNS的血管舒张效应无明显差异(P＞0.05)；但低氧高二氧化碳条件下，去内皮组的PNS血管舒张效应远大于内皮完整组(P＜0.05或P＜0.01)。结论:PNS可减轻低氧高二氧化碳性肺血管收缩，且去除肺动脉内皮后这种作用更加明显，其机制可能与内皮介导作用的缺失有关。【关键词】三七总皂甙低氧高碳酸血内皮细胞Abstract:Objective:ToinvestigatetheeffectofPanaxnotoginoside(PNS)onthemainpulmonaryarteryringsandtheunderlyingme

3、chanismsinvolvingintheroleofendothelialcell.Methods:Themodelofisolatedratpulmonaryarteryringsin11organbathswasperformed.Undernormoxiaandhypoxiahypercapniaconditions,theeffectofaccumulatedPNSonpulmonaryarteryringswhichwerepre-constrictedbyKC1ornorepinephrine(NE)wasobserved.Andtheendoth

4、eliumwasremovedinpartofthesepulmonaryarteryrings.Result:①Notonlyundernormoxiaconditionsbutalsohypoxiahypercapniaconditions,therewasconcentration-dependentrelaxationinpulmonaryarteryringspre-constrictedbyKC1（60mmol/L）,withorwithoutendothelium.Similarresultsoccurredinthosepulmonaryarter

5、yringswhichwerepre-constrictedbyNE(10-5mol／L)（P＜0.05）.②TherelaxationeffectofPNSonpulmonaryarteryringswasenhancedunderhypoxiahypercapniaconditions.Therewasstatisticallysignificantrelaxationineveryhypoxiahypercapniagroupcomparedwithnormoxiagroup(P＜0.05orP＜0.01).③Undernormoxiaconditions,

6、differentvaso-dilationdidnotoccurbetweenendothelium-denudedpulmonaryarteryringsandendothelium-integratedones(P＞0.05).Butunderhypoxiahypercapniaconditions,PNScauseddramaticallyrelaxationinendothelium-denudedpulmonaryarteryringsratherthanendothelium-integratedones(P＜0.05orP＜0.01).Conclu

7、sion:Underhypoxiahypercapniaconditions,PNScanrelaxtheratpulmonaryrings.Furthermore,inendothelium-denudedgroups,11dramaticallyrelaxationcausedbyPNSmayresultfromthedepletionofendotheliummediation.Keywords:Panaxnotoginoside;hypoxia;hypercapnia;endotheliumcells肺循环低氧状态（常同时伴有二氧化碳潴留）能诱发肺血管收缩

8、、肺血管阻