七叶皂苷钠对大鼠局灶性脑缺血再灌注后mmp9和ln表达的影响

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1、七叶皂苷钠对大鼠局灶性脑缺血再灌注后MMP9和LN表达的影响作者：张韶华，韩文江，李晓蕾，王秀丽【摘要】　　目的研究七叶皂苷钠(sodiumβaescin)对大鼠局灶性脑缺血再灌注后基质金属蛋白酶9(matrixmetalloproteinase9,MMP9)和层黏连蛋白(laminin,LN)表达影响，为深入探讨七叶皂苷钠对脑的保护机制提供实验依据。方法制备大鼠局灶性脑缺血再灌注模型，随机分为正常组、假手术组、缺血再灌注组和缺血再灌注七叶皂苷钠治疗组，后两组又分为缺血2h后再灌注3、6、12、24、72h和7d，共6个时间点，每时间点4只；用免疫组织化学法和图像分析

2、观察其缺血区LN和MMP9的表达变化。结果缺血再灌组MMP9在神经元的表达于再灌注6h开始增加，12h达高峰，24h又降低，7d最低，治疗组MMP9于再灌注同一时相有显著减少；LN在微血管的表达于再灌注12h明显减少，24h时达低峰，72h开始回升，治疗组在同一时相LN表达显著增多。结论MMP9与LN可能参与脑血管源性脑水肿的形成发展，缺血再灌注早期七叶皂苷钠治疗通过减少MMP9的表达，增加LN的表达对缺血再灌注脑起保护作用。【关键词】七叶皂苷钠；基质金属蛋白酶9；层黏连蛋白；脑缺血再灌注；大鼠　12　　ABSTRACT:ObjectiveTostudytheeff

3、ectofsodiumβaescinonthematrixmetalloproteinase9(MMP9)andlaminin(LN)expressioninratsafterfocalcerebralischemiaandreperfusion,andprovideexperimentalevidencefortherelationshipofMMP9andLNexpressionchangewiththeneuroprotectionofsodiumβaescin.MethodsTheSpraqueDawley(SD)ratmodeloffocalcerebra

4、lischemiaandreperfusionofcerebralmiddlearterywasprepared.Fiftysixratswererandomlydividedintothenormal,sham,ischemiaandreperfusion,aswellasischemiaandreperfusiontreatedwithsodiumβaescingroups.Thelattertwowerefurtherdividedintosixsubgroupsaccordingtoreperfusiontimeintervalof3h,6h,12h,24h,72

5、hand7dafterbrainischemia,with4ratsineachsubgroup.ImmunohistochemicalstainingandimageanalysiswereusedtomeasuretheexpressionofMMP9andLNintheischemicregion.ResultsTheexpressionofMMP9startedtoincreaseat6h,reachedthepeakat12h,decreasedin24h,andreachedtheloweston7dincerebralischemiaandreperfusio

6、ngroup.TheexpressionofMMP9inthesametimegroupsofthecerebralischemiaandreperfusiontreatedwithsodiumβaescingroupallobviouslydecreased(P<0.05).TheexpressionofLNinthe12microvesselsstartedtodecreaseat3h,reachedthelowestat24h,andincreasedat72hinthecerebralischemiaandreperfusiongroup.Theexpres

7、sionofLNinthesametimegroupsofthecerebralischemiaandreperfusiontreatedwithsodiumβaescingroupallelevated(P<0.05).ConclusionMMP9andLNprobablyhaveacloserelationshipwiththeformationandprogressionofvascularbrainedema.Sodiumβaescincanhelprecoveryof