vegf在大鼠缺血再灌注损伤肝组织中的表达及其意义

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1、VEGF在大鼠缺血再灌注损伤肝组织中的表达及其意义【摘要】目的：探讨血管内皮生长因子（vascularendothelialgrowthfactor，VEGF）在缺血再灌注损伤肝组织中的表达及意义。方法：利用雄性SD大鼠建立部分肝血流阻断动物模型，肝血流阻断90min后恢复血流灌注，再灌注后1,6h处死大鼠收集标本。分别采用全自动生化分析仪、HE染色，比色法、ELISA法测定血清转氨酶(ALT,AST)，肝组织病理改变,肝组织髓过氧化物酶（MPO）的活性以及VEGF蛋白的含量。结果：与假手术组相比，缺血再灌注组（I/R组）缺血肝脏MPO活性，肝组织内VEGF蛋白的含量以及ALT，

2、AST在再灌注1h即升高，且缺血再灌注6h时较1h明显升高；缺血肝脏病理切片可见大量炎症细胞浸润，6h炎症反应更为剧烈，部分肝细胞呈点状坏死。结论：VEGF蛋白表达的上调可能参与了大鼠肝脏缺血再灌注过程中肝脏的损伤。【关键词】肝脏；缺血；再灌注损伤；血管内皮生长因子　　［Abstract］Objective:Toinvestigatetheexpressionofvascularendothelialgrowthfactor(VEGF)intheliverischemia/reperfusioninjuryandsignificance.Methods：ThemaleSDrats

3、wereusedtoestablishpartialhepaticvascular9exclusionanimalmodel.Afterischemiafor90min，SDratsliverwerereperfused，andthenliverspecimenswerecollectedat1hand6hafterreperfusionrespectively.Routineassayswereperformedfortestingthelevelsofalanineaminotransferase(ALT),aspartateaminotransferase（AST）andt

4、heactivityofhepaticmyeloperoxidase(MPO).ThechangesofVEGFproteininischemicliverlobesweredeterminedbyELISA.Thelivertissuespathologicalchangeswereevaluatedinhematoxylinandeosin(HE)stained.Results：After90minofpartialliverischemia,theAST,ALTlevelinI/Rgroupwassignificantlyincreasedascomparedwiththe

5、sham-operated(SH)group,andAST,ALTlevelatthe6hreperfusionwasmarklyhigherthanthatatthe1hreperfusion.TheexpressionofVEGFproteinandtheMPOactivityinhepatictissueswereremarkablyup-regulatedinI/RgroupthanintheSHgroupatthetimepointof1hand6hreperfusion.Theywerehigherat6hreperfusionthanthatat1h.ForHEst

6、aining,theinfiltrationofmanyinflammatorycellswasobservedinlivertissueandbecamemuchsevererat6hposT-reperfusionlivertissue.Conclusion：Theup-regulationofVEGFproteininhepaticI/Rinjuryprocessmightbeinvolvedintheprocessofliverinjury.9　　［Keywords］liver；ischemia；reperfusioninjury；vascularendothelialg

7、rowthfactor　　缺血再灌注损伤是肝移植、复杂肝切除术中常常涉及的共同病理过程，多种细胞因子参与并介导了肝缺血再灌注损伤过程中的各个环节。近年来,国外许多学者研究发现血管内皮生长因子（vascularendothelialgrowthfactor，VEGF）与缺血再灌注损伤密切相关［1,2］。血管内皮生长因子又称血管通透因子(vascularpermeabilityfactor，VPF)，它是一种二聚糖，蛋白相对分子质量为34～45kD,由两个相同的亚基组成，是内皮细胞产