il1β511位点和tnfα308位点基因多态性与陕西汉族非贲门胃癌的关系

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1、IL1β511位点和TNFα308位点基因多态性与陕西汉族非贲门胃癌的关系作者：贾皑，龚均，厉英超，苌新明，郝志明，董蕾【摘要】目的探讨促炎症因子白细胞介素(IL)1β基因启动子区511位点及肿瘤坏死因子α(TNFα)308位点基因多态性与陕西汉族非贲门胃癌易感性的关系。方法用聚合酶链反应和限制性片段长度多态性(PCRRFLP)的方法对陕西汉族106例非贲门胃癌及108例非溃疡性消化不良患者(对照组)进行IL1β启动子的511位点和TNFα的308位点多态性分析。结果IL1β基因启动子511位点基因型CC、CT、TT频率在胃癌组和对照组分别为12.3%、54.7

2、%、33%和16.7%、50.9%、32.4%，无显著性差异(P>0.05)。等位基因C、T频率在胃癌组和对照组分别为39.6%、60.4%和42.1%、57.9%，其基因型频率和等位基因频率在胃癌组和对照组比较均无显著性差异(P>0.05)。TNFα的308位点的基因型GG、GA、AA频率在胃癌组和对照组分别为90.6%、1.9%、7.5%和84.3%、12.9%、2.8%，等位基因G、A频率在胃癌组和对照组分别为91.5%、8.5%和85.6%、14.4%，其基因型频率和等位基因频率在胃癌组和对照组比较均无显著性差异(P>0.05)。结论IL1β启动子511位点基

3、因多态性及TNFα308基因多态性与陕西汉族非贲门胃癌之间未发现相关关系。14【关键词】白细胞介素1；肿瘤坏死因子α；基因多态性；胃癌；汉族ABSTRACT:ObjectiveToinvestigatethecorrelationofthepolymorphismsoftheIL1βpromoterregion511C/Tandtumornecrosisfactorα(TNFα)308G/AgenewiththesusceptibilityofnoncardiacgastriccancerofShaanxiChinesepopulation.MethodsThepolymor

4、phismsintheIL1βpromoterregionandTNFαweredeterminedbyPCRRFLPinaHannationalityofShaanxiChinesepopulation.Totally106casesofnoncardiacgastriccancerand108casesofnonulcerdyspepsiaascontrolsweresubjectedtoIL1βandTNFαgeneanalysis.ResultsIL1βgenotypefrequenciesofCC,CTandTTwere12.3%,54.7%and33%ingastr

5、iccancergroup;16.7%,50.9%,32.4%incontrolgrouprespectively.AllelefrequenciesofCandTwere39.6%,60.4%ingastriccancergroup;42.1%and57.9%incontrolgrouprespectively.TNFα308genotypefrequenciesofGG,GAandAAwere90.6%,1.9%and7.5%ingastriccancergroup;84.3%,12.9%,2.8%incontrolgrouprespectively.Allelefrequencies

6、ofGandAwere91.5%and8.5%ingastriccancergroup;85.6%and14.4%incontrolgrouprespectively.Therewerenosignificantdifferencesintheallelefrequencyandgenotypeof14IL1β511C/TandTNFα308(P>0.05).ConclusionNeitherthe511C/TpolymorphismintheIL1βpromoterregionnorTNFα308genepolymorphismwasassociatedwithsusc

7、eptibilityofnoncardiacgastriccancerinaHannationalityofShaanxiChinesepopulation.KEYWOEDS:interleukin1β;tumornecrosisfactorα;genepolymorphism;gastriccancer;Chinesepopulation　胃癌是人类最常见的恶性肿瘤之一，陕西是胃癌的高发区