cck-8对lps诱导大鼠tasmcs cuzn-sod基因表达影响的受体机制

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1、CCK-8对LPS诱导大鼠TASMCsCuZn-SOD基因表达影响的受体机制作者：董国凯,谷振勇,马丽琴,李娜,马春玲,丛斌【摘要】目的探讨八肽胆囊收缩素(CCK-8)对体外脂多糖(LPS)诱导大鼠胸主动脉平滑肌细胞(TASMCs)CuZn-SOD基因表达影响的受体机制。方法用贴块法培养大鼠TASMCs，经CCK-8、LPS、CR-1409、CR-2945、CCK+LPS、CR-1409+LPS、CR-2945+LPS、CR-1409+CCK-8+LPS、CR-2945+CCK-8+LPS及溶剂单独或共同孵育细胞，用RT-PCR技术检测CuZn-SODmRNA表达。结果对照

2、组TASMCsCuZn-SODmRNA低水平表达，LPS诱导TASMCsCuZn-SODmRNA表达上调(P<0.05)，CCK-8预处理后，LPS对TASMCsCuZn-SODmRNA表达的诱导作用可部分受抑，预先用CR-1409（1×10-5mol/L）、CR-2945（1×10-5mol/L）处理后，再分别加入LPS和CCK-8，CR-1409+CCK-8+LPS、CR-2945+CCK-8+LPS两组CuZn-SODmRNA表达均高于CCK-8+LPS组（P<0.01），但仍低于LPS组(P<0.05)，其中CR-2945的拮抗作用比CR-1409

3、稍明显;CR-1409、2945单独作用与对照组相比差异无显著性(P>0.05)。结论CCK-8通过其受体下调LPS对TASMCsCuZn-SOD基因表达的诱导作用，其中CCK-BR较CCK-AR作用稍大。【关键词】11八肽胆囊收缩素；脂多糖；血管平滑肌细胞；CCK受体；铜-锌超氧化物歧化酶　　Abstract：ObjectiveToinvestigatetheeffectofCCK-8receptoronLPS-inducedCuZn-SODmRNAexpressioninratTASMCs.MethodsRatthoracicaortasmoothmusclece

4、lls(TASMCs)fromtheattachment-blockculturewereincubatedwithNS,LPS(0.1mg/L),CCK-8(1×10-8mol/L),CR-1409(1×10-5mol/L,thespecificantagonistofCCK-AR)andCR-2945(1×10-5mol/L,thespecificantagonistofCCK-BR),respectivelyorincombination.TheexpressionsofCuZn-SODmRNAwereassayedbyRT-PCR.ResultsTheexpress

5、ionsofLPS-inducedCuZn-SODmRNAwereup-regulated,comparedwiththecontrolgroup(P<0.05).SubsequenttoCCK-8pretreatment,theinductionofLPStotheexpressionsofCuZn-SODmRNAinTASMCswaspartiallyinhibited.FollowingpretreatmentwithCR-1409(1×10-5mol/L)andCR-2945(1×10-5mol/L),LPSandCCK-8wererespectivelyad

6、ded.TheexpressionsinCR-1409+CCK-8+LPSandCR-2945+CCK-8+LPSgroupswerehigherthanCCK-8+LPSgroup(P<0.01),butwerestilllowerthanthoseofLPSgroup(P<0.05).TheantagonisteffectofCR-2945wasslightlymoreevidentthanthatofCR-1409,whileeitherCR-1409orCR-2945alonehadnosignificantdifferencesincomparison

7、withthecontrolgroup(P>0.05).ConclusionCCK-811candown-regulateLPS-inducedCuZn-SODmRNAexpressioninTASMCsbymeansofitsreceptorsCCK-ARandCCK-BR,ofwhichCCK-BRhadslightlygreaterinductionthanCCK-AR.　　Keywords:cholecystokininoctapeptide;lipopoly-saccharides;thoracic