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1、圆背角无齿蚌中抗肿瘤活性组分的提取工艺研究中草焉ChineseTraditionalandHerbalDrugs第38卷第6期2007年6月?839?[4]Es]I-6]testinalepithelialcellline:Caco一2rJ1.ActaPharmacolSin,2003,24(12):1185—1191.ZhaoXC,WangGJ,SunBW,eta1.Changesofexpressionandfunctionsofdipeptidetransporterafteranoxia/reoxy—genationinCaco一2ceilsFJ].JChinaP
2、harmUniv(中国药科大学),2003,34(1):74—77.JiangXH,JiaYT,YuanY,eta1.TheapplicationofCaco一2cellmodelindrugoralabsorptionFJ].ChinPharmJ(中国药学杂志),2002,37(5):325—327.HaberBA,MohnKL,DiamondRH,eta1.Inductionpat—ternsof70genesduringninedaysafterhepaterctomydefinethetemporalcourseofliverregenerationrJ1.JCl
3、inInvest,1993.91:1319—1341.[73ArturssonP,KarlssonJ.Correlationbetweenoraldrugab—sorptioninhumansandapparentdrugpermeabilitycoeffi—cientsinhumanintestinalepithelial(Caco一2)cells[J].BiochemBiophysResCommun,1991,175(3):880—885.E83EarvinL,JohnP,MehranY.Mechanismsoftransportandstructure—permea
4、bilityrelationshipofsulfasalazineanditsanalogsinCaco一2cellmonolayersFJ].PharmRes,2000,17(10):1168—1174.[9]SatioH,InuiKI.Dipeptidetransportersinapicalandbaso—latera1membranesofthehumanintestinecel11ineCaco一2rJ1.AmJPhysiol,1993,265:289—293.圆背角无齿蚌中抗肿瘤活性组分的提取工艺研究柯乾坤,胡水根,刘娟,陈正伟,卞俊,王朝武,蔡定国(1.解放
5、军第四一一医院南京军区药学专科中心,上海200434;2.江西中医学院,江西南昌330006)摘要:目的优选圆背角无齿蚌中有效组分的最佳提取工艺.方法选用L.(3)正交试验,以水提次数,醇沉体积分数,盐溶的氯化钠质量浓度为因素进行比较,对影响圆背角无齿蚌中有效组分的提取工艺进行优选.采用硫酸一苯酚法和凯氏定氮法测定含糖量和含氮量.以综合加权评分法比较实验结果.结果最佳提取工艺为在4℃以下用4倍蒸馏水量,提取1次,醇沉时的乙醇体积分数为7O%,盐析的氯化钠溶液质量浓度为8%.结论优选的圆背角无齿蚌中有效组分的提取工艺可行.关键词:圆背角无齿蚌;正交试验;硫酸一苯酚法;凯式定
6、氮法中图分类号:R286.1文献标识码:A文章编号:0253—2670(2007)06—0839—03ExtractiontechniquesofantitumoreffectveconstituentfromodianaanatontaKEQian—kun,HUShui—gen,LIUJuan,CHENZheng—wei,BIANjun,WANGChao—wu.CAIDing—guo(1.ResearchCenterofPharmacy,411HospitalofPLA,Shanghai200434,China;2.JiangxiCollegeofTraditional
7、ChineseMedicine,Nanchang330006,China)Abstract:ObjectiveTooptimizetheextractionofeffectveconstituentfromWoodianaanatonta.MethodsLg(3)Orthogonaltestwasusedwiththreefactors,includingtimesofwaterextracting,con-centrationofethanolprecipitation,andconcentrationofsodiumchl
