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1、1降低神经病理性疼痛大鼠海马和PAGIL-6mRNA的表达第44卷第10期2006年10月山东大学(医学版)JOURNALOFSHANDONGUNWERSffY(HEALTHSCIENCES)V01.44No.100et.20o6文章编号:1671—7554(2006)10—1028一o4侧脑室注射内吗啡肽一1降低神经病理性疼痛大鼠海马和PAGIL一6mRNA的表达孙涛,宋文阁,姚尚龙,傅志俭(1.山东省立医院疼痛科,山东济南250021;2.华中科技大学同济医学院附属协和医院麻醉科,湖北武汉430022)[摘要]目的:观察侧脑室微量注射内吗啡肽.1(endomorphin.1,EM.1
2、)对坐骨神经慢性压迫性损伤(chronicconstrictioninjury,CCI)大鼠痛阈以及海马和中脑导水管周围灰质(periaqueductalgray,PAG)中白细胞介素.6(interleukin.6,IL-6)mRNA表达的影响.方法:建立大鼠慢性坐骨神经压迫性损伤模型,侧脑室微量注射EM.1(20g/20),手术前1d和手术后第7d观察其机械性痛敏和热痛敏的变化,术后第7d将大鼠处死,采用原位杂交观察海马和PAGIL-6mRNA的表达,假手术及侧脑室注射生理盐水作对照.结果:实验发现CCI手术导致大鼠机械性痛阈和热痛阈明显下降,且增加大鼠海马和PAG部位IL-6mRN
3、A的表达.侧脑室注射EM.1可提高大鼠基础痛阈,且显着改善大鼠神经病理性疼痛状态,降低海马和PAG部位IL-6mRNA的表达.结论:侧脑室微量注射EM.1可有效抑制神经病理性疼痛,且明显降低海马和PAGIL-6的表达.[关键词]慢性压迫性损伤;内吗啡肽.1;白细胞介素6[中图分类号]R614[文献标识码]ADown?-regulationofinterleukin?-6mRNAinhippocampusandofPAGinchronicneuropathicpainmodelofratsafterintracerebroventricularmicroinjectionofendomor
4、phin一1SUNTao,SONGWen.ge,YAOShang.1ong2,FUZhi.jian(1.DepartmentofPainManagement,ShandongProvincialHospital,Jinan250021,Shandong,China;2.DepartmentofAnesthesiology,UnionHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuhan430022,Hubei,China)[ABSTRACT]Objective:Toinvestigateth
5、eeffectofintracerebroventricular(i.c.v.)injectionofendomorphin-1(EM-1)onIL-6mRNAexpressionlevelinhippocampusandonPAGinchronicconstrictioninjury(CCI)modelofrats.Methods:TheCCImodelwasestablishedandgivenendomorphin-1(20∥20,i.C.v)oncedailyfor3consec-utivedays.ThecontrolratswereoperatedonandinjectedN
6、S.Themechanicalandthethermalpainthresholdweremen-sured1daybeforeoperationand7daysafteroperation.TheexpressionlevelofIL-6mRNAWasmeasuredbyinsituhy-bridization.Results:MechanicalallodyniaandthermalhyperalgesiawereinducedinCCIrats.Inaddition,theexpres-sionlevelofIL-6mRNAinhippocampusandPAGmarkedlyin
7、creased.IntracerebmventricularinjectionofEM-1in-creasedthebasicpainthresholdoftherats,reversedtheCCI-inducedexistingneuropathicpainstateandsignificantlysuppressedtheincreasedexpressionlevelofIL-6mRNAinducedbyCCI.Conclu
