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ID:14711759
大小:249.00 KB
页数:7页
时间:2018-07-30
《冰片与顺铂联用对eca109(ddpr)细胞增殖、细胞周期和凋亡》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、冰片与顺铂联用对Eca109(DDPr)细胞增殖、细胞周期和凋亡的影响戴翠萍,张徐宁,薛彩萍,季宁东[基金项目]:江苏省淮安市科技支撑计划项目(HAS2009002-2);江苏省中医药局科技项目(LB09077)*通讯作者:E-mail:hywxjnd1@163.com(淮阴卫生高等职业技术学校,淮安市消化道肿瘤重点实验室,江苏淮安223300)[摘要]目的:探讨冰片与顺铂(cisplatin,DDP)联用对耐DDP的食管癌细胞Eca109(DDPr)增殖、细胞周期和凋亡的影响。方法:采用递增药物质量浓度持续作用诱导法建立耐DDP
2、的食管癌细胞Eca109(DDPr)。冰片联合DDP作用于Eca109(DDPr)细胞,MTT法检测Eca109(DDPr)细胞增殖情况;流式细胞术检测细胞周期及细胞凋亡。结果:递增药物质量浓度持续作用诱导6个月后,细胞可以在含1μg/mlDDP的培养液中正常生长,其对DDP的耐药指数为15.886,所得细胞命名为耐DDP的食管癌细胞Eca109(DDPr)。单用冰片浓度≤1.560μg/ml时,对Eca109(DDPr)细胞无明显抑制作用(P>0.05),但与DDP1μg/ml合用能显著促进DDP抑制Eca109(DDPr)细胞
3、的增殖(P<0.05),并促进DDP诱导Eca109(DDPr)细胞周期改变、细胞凋亡增加。细胞周期表现为G1期细胞明显增多(P<0.05),S期细胞、G2期细胞明显减少(P<0.05);细胞的凋亡率明显增加(P<0.05)。结论:冰片促进DDP抑制Eca109(DDPr)细胞增殖可能与改变Eca109(DDPr)细胞周期、增加细胞凋亡有关。[关键词]冰片;顺铂;Eca109(DDPr);细胞增殖;细胞周期;细胞凋亡[中图分类号]R735.1[文献标识码]AEffectsofBorneolcombinedwithcisplatin
4、onCellProliferationandCellCycleandApoptosisinHumanEsophagealCarcinomaCelllineEca109(DDPr)DAICui-ping,ZHANGXu-ning,XUECai-ping,JINing-dong(HuaiyinAdvancedVocationalandTechnicalHealthSchool,HuaianKeyLaboratoryoftheTumorofDigestiveSystem,Huaian223300,China)[Abstract]Obje
5、ctive:Toexploretheeffectsofborneolcombinedwithcisplatin(DDP)onCellproliferationandcellcycleandapoptosisinhumanEsophagealCarcinomacelllineEca109(DDPr).Methods:ThehumanesophagealcarcinomacelllineEca109wasexposedtocontinuousstepwise-increasedconcentrationofDDP.Drug-sensi
6、tivitywasmeasuredbyMTT.Thecellcycleandapoptosisofcellsweredeterminedbyflowcytometry.Results:After6monthsstepwisedrugexposure,thecellcouldnormallygrowandpropagateinthemediumwith1μg/mlofDDP,Itexhibitedaresistanceindexof15.886againstDDP.ThecelllinewasnamedEca109(DDPr).MT
7、Tassayshowedthatborneolattheconcentrationnomorethan1.560μg/mlhadnoobviousinhibitiontoEca109(DDPr)cells(P>0.05),butitcouldsignificantlypromoteDDP(1μg/ml)toinhibittheproliferationofEca109(DDPr)cells(P<0.05).ComparedwithusingDDPalone,BorneolcombinedwithDDPcouldchangethec
8、ellcycle,significantlyincreasethenumberofcellsinG1stage(P<0.05)anddecreasethenumberofcellsinSstageandG2stage(P<0.05),andobvi
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