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1、经血源性子宫内膜间充质干细胞的分离、培养与鉴定中国组织工程研究与临床康复彩74誉32助2010—08—06出版JournalofClinicalRehabilitativeTissueEngineeringResearchAugust6.2010Vo1.14,No.32经血源性子宫内膜间充质干细胞的分离,培养与鉴定冰术术米水★周云帆,杨波'',胡祥'',焦洪亮.',周长辉',田毅...,雷宁静',谷晨熙.,许予I1J】,戴建武.,关方霞'Isolation,cultureandidentificationofmenstrualblood-derivedmesenchymalste
2、mcellsZhouYun-f叩'一,YangBo一,HuXiang一,JiaoHong.1iang一.ZhouChang.hui',TianYi一,LeiNing-jing',GuChen---xi,XuYu—mingDaiJian.wu.GuanFang-xia.'DepartmentofBioangineedng,ZhengouUn'wets~ty.Zhangzhou450001.HenanProvince.China;OpenLaboratoryofClinicaIMedicineandKeySubject,HenanlnstitutionofHigherEduceU~,
3、Zhengzhou450052.HenanProvince.China;Depar~entofNeurosurge~.FirslAffiliatedHospita1.~angzhouUniversity.Zhengzhou450052HensnProvince.China;JiangsuPublicTechnologySe~cePlatformofStemCelIsandBio~herapy,'hou225300JiangsuProvince.China;.ShenzhanBeikeCalIEngineeringInstJtute.Shanzhan518O00GuangdoogP
4、rovince.China;DDeparlmentofCliniceIMedicine,angzhouUniversity.Zhangzhou45o001.HenanProvince.China;DeparlmentofNeurology.FlrstatedHospital,ZhangzhouUniversi,Zheogzhou450052HenanProvince.China;lntuteofGane~csandDevelopmentalBiology.ChineseAcademyofSciences.Beijing100190.ChinaZhouJn-fan★.Studyin
5、gformaster'sdegree,DepartmentofBicengineedng.ZhangzhouUniversity,Zhengz'hou450~1.HenanProvince.China;OpenLaboratoryofClinicaIMedicineandKeySubject,HenanInstitutionofHigherEducation,Z.hangzhou450052,HenanProvince.Chinaboat-1986@163.COWl5952AbstractBACKGR0UND:Generally.themainsourcesofstemcells
6、areembryonicstemcellsandbonemarrowstemcellsbutIherearesomelimitationsofthetwokindsofsources,andresearchersstrivetofindanewtypeofstemcellsinordertoovercomethepotentialforteratoma,thelackofsamplesources.ethicscontroversiesandsoon.OBJECTIVE:Toisolate,cultureandidentifymenstruaIblood—derivedmesen
7、chymalstemcells(MMCs).METHODS:MMCswereseparatedbvFicolIdensitygradientcentrifugation.CelImorphologyandgrowthcharacteristicswereobserved.Flowc~ometryanalysiswasusedtoidentifyexpressionofCD29,CD44,CD34,CD45,HLA-ABCandHLA-DRsurfacemarkersinMMCs.
