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1、咖啡因和维生素E对小尾寒羊精液冷冻保存的影响中国蓉学c亟握2010,26(8):l一6ChineseAgriculturalScienceBulletin咖啡因和维生素E对小尾寒羊精液冷冻保存的影响崔旭,罗桂河.,李卫东.,李复煌,孟丹娜,吕建伟,穆笛,倪和民(北京农学院动物科学技术系,北京102206;北京市昌平区动物卫生监督管理局,北京102200;北京市昌平区动物卫生防疫站,北京102200;北京市畜牧兽医总站,北京100107)摘要:研究冷冻稀释液中分别添加不同浓度的咖啡因和维生素E后对小尾寒羊精液冷冻保存的影响.将不同浓度的咖啡因和维生素E分别添加于冷冻前的小尾寒羊精液稀释液中,
2、用精子活率,顸体完整率和畸形率,作为判定精子冷冻品质的指标,初步确定2种稀释液添加成分对小尾寒羊精液品质的影响.当添加维生素E的浓度为0.4mg/mL时,冷冻后的精子活率较高,畸形率较低,且精子顶体完整率也较高;添加5mg/mL咖啡因能显着提高精子的解冻后活率,其活率为0.35,对精子畸形率和顶体完整率无显着影响.在冷冻精液稀释液中添加适当浓度维生素E可以减缓冷冻对精子的伤害,提高小尾寒羊冷冻精子活率,改善冷冻精液的品质;当添加5mg/mL的咖啡因于稀释液中冷冻时,解冻后精液活率显着提高,但不影响精子畸形率和顶体完整率.关键词:咖啡因;维生素E;精液;小尾寒羊中图分类号:S814.8文献标
3、志码:A论文编号:2009—2101EffectofDifferentConcentrationsofCaffeine,VEontheFreezingSemenofSmall—tailedHanSheepCuiXu,LuoGuihe,LiWeidong3,LiFuhuang4,MengDanna,LtiJianwei,MuDi,NiHeroin(DepartmentofAnimalScience&Technology,BengUniversityofAgriculture,Beijing102206;2AnimalHealthandHygieneAdministrationBure
4、auofBengChangpingDistrict,Beijing102200;3AnimalHealthandHygieneAdministrationStationofBeijingChangpingDistrict,Beijing102200;"Be~iingGeneralStationofAnimalHusbandryandVeterinaryMedicine,Beijing100107)Abstract:Thisstudywasaimedtoinvestigatetheeffectofdifferentconcentrationsofcaffeine,vitaminE(VE)on
5、thefreezingsemenofsmalltailedHansheep.ThedifferentconcentrationsofcaffeineandVEwereaddedtothepre-frozensheepsemendiluentinsmall-tailedHansheep,thenthemotilityrateoftheirpost-thawedse—men,thepercentageofspermswithintactacrosome,andthepercentageoftheirabnormality,wererespectivelyestimatedtodetectthe
6、qualityoftheirfrozensemen.Theresultswereshownasfollowing:Whiletheconcentra—tionofVEwas0.4mg/mL,afterfreezingandthawing,thelivabilityrateofthesemenfromsmalltailedHansheepishigh,whereastheirabnormalityrateislow,buttheapicalbodypercentageofspermheadisstillhigh.Meanwhile,addingwith5mg/mLcaffeineinthei
7、rfreezingdiluents,canalsoobviouslyimprovetheirspermliva—bilityrate(0.35),butnotaffecttheirabnormalrateandtheapicalbodyrate.AddingappropriateconcentrationsofvitaminEtothefrozensemendiluent,canslowthefreezingofsper
