pcr protocols 2d ed - john m. s. bartlett

《pcr protocols 2d ed - john m. s. bartlett》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、MethodsinMolecularBiologyTMVolume226PCRPCRProtocolsProtocolsSSECONDECONDEEDITIONDITIONEditedbyEditedbyJohnM.S.BartlettJohnM.S.BartlettDavidStirlingDavidStirlingContents1.AShortHistoryofthePolymeraseChainReaction3JohnM.S.BartlettandDavidStirling2.PCRPatentIssues7Pete

2、rCarrollandDavidCasimir3.EquippingandEstablishingaPCRLaboratory15SusanMcDonagh4.QualityControlinPCR20DavidStirling5.ExtractionofNucleicAcidTemplates27JohnM.S.Bartlett6.ExtractionofDNAfromWholeBlood29JohnM.S.BartlettandAnneWhite7.DNAExtractionfromTissue33HelenPearson

3、andDavidStirling8.ExtractionofDNAfromMicrodissectedArchivalTissues35JamesJ.Going9.RNAExtractionfromBlood43HelenPearson10.RNAExtractionfromFrozenTissue45JohnM.S.Bartlett11.RNAExtractionfromTissueSections47HelenPearson12.DualDNA/RNAExtraction49DavidStirlingandJohnM.S.

4、Bartlett13.DNAExtractionfromFungi,Yeast,andBacteria53DavidStirling14.IsolationofRNAVirusesfromBiologicalMaterials55SusanMcDonagh15.ExtractionofAncientDNA57WeraM.Schmerer16.DNAExtractionfromPlasmaandSerum63DavidStirling17.TechnicalNotesfortheDetectionofNucleicAcids65

5、JohnM.S.Bartlett18.TechnicalNotesfortheRecoveryandPurificationofPCRProductsfromAcrylamideGels77DavidStirling19.PCRPrimerDesign81DavidL.HyndmanandMasatoMitsuhashi20.OptimizationofPolymeraseChainReactions89HaiyingGrunenwald21.SubcyclingPCRforLong-DistanceAmplification

6、sofRegionswithHighandLowGuanine–CystineContent----AmplificationoftheIntron22InversionoftheFVIIIGeneDavidStirling10122.RapidAmplificationofcDNAEndsiXinWangandW.ScottYoungIII10523.RandomlyAmplifiedPolymorphicDNAFingerprinting--TheBasics117RanilS.DassanayakeandLakshman

7、P.Samaranayake24.Microsphere-BasedSingleNucleotidePolymorphismGenotyping123MarieA.Iannone,J.DavidTaylor,JingwenChen,May-SungLi,FeiYe,andMichaelP.Weiner25.LigaseChainReaction135WilliamH.Benjamin,Jr.,KimR.Smith,andKenB.Waites26.NestedRT-PCRinaSingleClosedTube151Antoni

8、oOlmos,OlgaEsteban,EdsonBertolini,andMarianoCambra27.DirectPCRfromSerum161kenjiAbe28.LongPCRAmplificationofLargeFragmentsofViralGenomes167