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1、M™ethodsinMolecularBiologySeriesEditorJohnM.WalkerSchoolofLifeSciencesUniversityofHertfordshireHatfield,Hertfordshire,AL109AB,UKForothertitlespublishedinthisseries,gotowww.springer.com/series/7651InVitroMutagenesisProtocolsThirdEditionEditedbyJeffBramanStratagene,AnAgilentTechnologies
2、Division,LaJolla,CA,USAEditorJeffBraman,Ph.D.StratageneAnAgilentTechnologiesDivisionLaJolla,CAUSAjeff.braman@agilent.comISSN1064-3745e-ISSN1940-6029ISBN978-1-60761-651-1e-ISBN978-1-60761-652-8DOI10.1007/978-1-60761-652-8SpringerNewYorkDordrechtHeidelbergLondonLibraryofCongressControlN
3、umber:2010925108©SpringerScience+BusinessMedia,LLC2010Allrightsreserved.Thisworkmaynotbetranslatedorcopiedinwholeorinpartwithoutthewrittenpermissionofthepublisher(HumanaPress,c/oSpringerScience+BusinessMedia,LLC,233SpringStreet,NewYork,NY10013,USA),exceptforbriefexcerptsinconnectionwi
4、threviewsorscholarlyanalysis.Useinconnectionwithanyformofinformationstorageandretrieval,electronicadaptation,computersoftware,orbysimilarordissimilarmethodologynowknownorhereafterdevelopedisforbidden.Theuseinthispublicationoftradenames,trademarks,servicemarks,andsimilarterms,evenifthe
5、yarenotidentifiedassuch,isnottobetakenasanexpressionofopinionastowhetherornottheyaresubjecttoproprietaryrights.Whiletheadviceandinformationinthisbookarebelievedtobetrueandaccurateatthedateofgoingtopress,neithertheauthorsnortheeditorsnorthepublishercanacceptanylegalresponsibilityforany
6、errorsoromissionsthatmaybemade.Thepublishermakesnowarranty,expressorimplied,withrespecttothematerialcontainedherein.Coverillustration:BasedonFigure2AofChapter16Printedonacid-freepaperHumanaPressisapartofSpringerScience+BusinessMedia(www.springer.com)PrefaceIntheprefacetothesecondediti
7、onofthisvolume,Iclaimedthatthebookrepresentedatoolboxcontainingprotocolstoadvancetheunderstandingoftheconnectionbetweennucleotidesequenceandsequencefunction.Thesameholdstrueinthisthirdedition,withanotableexception;thethirdeditioncontainsavarietyofspecialtytoolssuccessfullyemployedbysc
8、ienti
