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1、YangHetong,TangWenhua,MaartenRyder,LiJishun,GuoYong,ZhouHongzi.2004.LectinsoffungalpathogensaspotentialtoolsinselectingpromisingbiologicalcontrolagentsofTrichodermaspp.J.ShandongUniversityofTechnology(Sci&Tech),18(6):15-23Lectinsoffungalpathogensaspotentialtoolsinsel
2、ectingpromisingbiologicalcontrolagentsofTrichodermaspp.YangHetong1,2,3,4,TangWenhua2,MaartenRyder3,LiJishun1,GuoYong1,ZhouHongzi1(1BiologyResearchInstituteofShandongAcademyofSciences,Jinan,250014;2Dept.PlantPathology,ChinaAgriculturalUniversity,Beijing,China,100094;3
3、AdelaideLab,CSIROLandandWater,GlenOsmond,SA5064,AdelaideAustralia;4LifeScienceCollege,ShandongUniversityofTechnology,Zibo,255049)Abstract:Inthisarticle,lectinsof8pathogenstrainswereextractedandpurified.Bytestingagglutinationlectinswith19Trichodermaspp.srains,alllecti
4、nsfromfungiagglutinatedtheconidiaofTrichodermaspp.withdifferenttiters,exceptlectinfromAlternariasolaniMs.ResultindicatedthattheagglutinationofTrichodermaconidiabyfungallectinswasrelatedtotheireffectivenesscausedbythecorrespondingpathogens.Statisticalanalysisshowedsig
5、nificantcorrelationbetweentitersofFusariumlectinandtheeffectivenessofTrichodermaagainstthedisease.TheagglutinationtestmightbeappliedtopredictpotentialeffectivenessofagivenTrichodermastrainagainstthecorrespondingdiseaseandthelectinscouldbeemployedinscreeningTrichoderm
6、asppasbiocontrolagentsefficiently.Keywords:lectins;pathogens;agglutination;TrichodermasppAsuitablemethodwascrucialforobtainingefficientbiocontrolmicrobialantagonistsinsoilborneplantdiseasecontrol.Thestrategiesadoptedbymostresearchersatpresentinscreeningwerevarious(So
7、ngetal,1996),butthereweretwobasicones:(1)themostfrequentlyadoptedonewastotesttheinhibitoryeffectincludingantibiosis,mycoparasitismandpathogenlysisofcandidatesonplateandthentesttheefficacyingreenhouseofthemostinhibitorymicroorganisms,followedbyextensiontofieldtrials.B
8、ythisstrategy,anumberofpotentialantagonistswasselectedfromalargenumberofcandidates,andthoseisolateswhichwerelessinhibitoryweredisca
