滤纸条与吸潮纸尖采集龈沟液样本比较

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1、滤纸条与吸潮纸尖采集龈沟液样本比较滤纸条与吸潮纸尖采集龈沟液样本比较滤纸条与吸潮纸尖采集龈沟液样本比较滤纸条与吸潮纸尖采集龈沟液样本比较滤纸条与吸潮纸尖采集龈沟液样本比较滤纸条与吸潮纸尖采集龈沟液样本比较滤纸条与吸潮纸尖采集龈沟液样本比较现代口腔医学杂志2008年第22卷第2期JModemStomatol,March2008,V0l22,No.2?137?滤纸条与吸潮纸尖采集龈沟液样本比较陈智滨孙晓军栾庆先【摘要】目的比较吸潮纸尖与传统滤纸条采集龈沟液样本质和量的差异,为临床选择适宜的采样材料提供实验依据.方法实验组(A组)吸潮纸尖,对照组(B组)传统滤纸条,手工裁剪成10×2ram长

2、方形.体外实验:质控血清20个递增体积分别加在两种材料上,同体积重复4次.分析体积与重量之问的关系;检测比较两种材料洗提液中四种成份回收率差异.体内实验:两种材料问隔三天采集牙周病患者同一位.占’样本龈沟液66份,计算样本的重量和体积范围,检测比较样本洗提液中的白细胞介素一6(interlenkin一6,lL一6)(n=27),白细胞介素一1B(interleukin—lp,IL—lB)(n=50)含量.结果体外试验结果秩和检验分析两组洗提液中4种成份的回收率>90%,且组间无显着?j生差异(P>0.05).体内实验组问龈沟液净重高度相关关系(r=0.959)且无显着性差异

3、(P>0.05).两组材料洗提液中IL—lB和IL一6统计无显着?j生差异P>0.05.结论实验结果证明吸潮纸尖可替代传统手工裁剪滤纸条,适于国内龈沟液研究工作.【关键词】龈沟液滤纸条吸潮纸尖Comparethedi骶rencebetweenthef’dterpaperandabsorbentpaperpointsinconecCmgGCFCHENZhibln,SUNX~ojun,LUANQingxian.DepartmentofPeriodontology,PekingUniversitySchoolandHospitalofSmrm~logy,BeijinglO0o8l

4、【Abstract】ObjectiveTocomparethedifferenceofabsorbentpaperpointsandwhatman3stripfilterpaperincollectingGCFandaimofselectingthesuitablesamplingmateria1.MethodsAbsorbentpaperpointswereusedasexperimentalgroupandwhatman3stripfdterpaperascontrolgroup.Thepapemwerecutinto10×2mmrectanglemanually.Invitros

5、tudy.TheSerlllnwasaddedontothetwomaterialstlIincrementalvolume.TheprocedurewasrepeatedfourtimesWitlIthesamevolume.Therelationshipbetweentheweightandvolume,aswellaSthedifferenceamongrecoveryratesoffourcomponentsintheelutionwereanalyzed.Invivostudy,GCFsamplesfromperiodontalpatientswerecollectedwit

6、h3daysintervalbyusingthetwomaterialsrespectively.Theweightandvolumeofthesampleswerecalculatedandcontentsoftheinterleukin-6(IL一6)andtheinterleukin一113(IL一113)intheelutionwerecompared.ResultsInvitrostudy,nostatisticallysignificantdifference(P>0.05)wasshownbetweenthetwogroupsintherecoveryratesof

7、thefourcomponents.Invlvostudy,nostatisticallysignificantdifference(P>0.05)WaSshownbetweentwogroupsinGCFweightandcontentsofIL一6andIL一113intheelutlon.Highcorrelation(r=0.959)wasfoundbetweentwogroupsinGCFweight.ConclusionThe