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1、第六卷第四期铜仁职业技术学院学报(自然科学版)Vol.6.No.42008年8月JournalofTongrenVocational&TechnicalCollege(NaturalScienceEdition)Aug.2008山羊抗小鹅瘟高免血清的制备及应用王文强122,王文成,熊凤兰(1.铜仁职业技术学院生物工程系,贵州铜仁554300;2.铜仁市畜牧兽医局,贵州铜仁554300)摘要:小鹅瘟是雏鹅感染鹅细小病毒(Gooseplaguevirus,GPV)所引起的一种急性败血型传染病,病程短且
2、死亡率很高,主要侵害1月龄以内的雏鹅和雏番鸭。目前,市场销售的对此病进行免疫和治疗的抗小鹅瘟血清存在产量低、成本高,且容易导致某些鹅源疫病的传播的缺点。笔者采用从贵州铜仁市等地的养鹅场采集的病鹅分离出的小鹅瘟野生强毒株接种于9日龄的鹅胚尿囊液制备了小鹅瘟病毒抗原,并用小鹅瘟病毒抗原加弗氏佐剂后,分多次皮下注射结合静脉注射小鹅瘟病毒抗原免疫贵州黑、白山羊。颈动脉一次性放血制备抗小鹅瘟异源高免血清,用琼脂扩散进行效价的测定,结果显示其抗体效价平均都在1∶8以上。将制备的血清与市售的抗小鹅瘟进行了对比试
3、验,结果市售的抗小鹅瘟血清的保护率为85%,山羊抗小鹅瘟高免血清的保护率为95%。山羊抗小鹅瘟异源高免血清具有效价高、成本低、安全性好等优点。关键词:小鹅瘟;山羊抗小鹅瘟高免血清;抗小鹅瘟血清;对比试验中图分类号:S854.5+7文献标识码:B文章编号:310-(2008)04-0001-(06)StudyandApplicationofHigh-ImmunizedSerumfromGoatAgainstGoslingPlague122WANGWen-qiang,WANGWen-cheng,XIO
4、NGFeng-lan(1.DepartmentofBiology&Engineering,TongrenVocational&TechnicalCollege,Tongren554300,Guizhou;2.BureauofAnimalHusbandryofTongrenCity,Tongren554300,Guizhou)Abstract:GoosePlague(GP),anacuteinfectiousdiseaselostbloodtypeingooes,wascausedbygoosepa
5、rvovirus(GPV),Shortcourseofthediseaseandhighmortality,mainlyagainstthelessthan1montholdandyounggoslingsMuscovy.Followingthe10-day-oldgoslingsmorbidity,mortalityratecouldbeashighas100percent.Atpresent,thatanti-plagueserumonGooseinmarketwasusedinmanyGoo
6、sefarmwasimmunizedandtreatedtoGosling.However,itislow-yield,highcost,andeasilyleadtothediseaseofgeesesourcespread..Inthispaper,thewildgoslingplagueXWhighlyvirulentstrainsofvaccinationthatwasisolatedfromTongren,GuizhouandotherplacesGoosefarm,.goslingpl
7、aguevirusantigenwaspreparedinthe9-day-oldembryogooseallantoicfluidwiththeVirus,gooseparvovirusantigenaddedwithFreund,scompleteAdjuvantwasusedtoimmunizedgoatsofguizhouwithseveralspotssubcutaneousandvieninjection.serumofanti-alienGoosePlagueHigh-immuniz
8、edwascollectedinCarotidarterybleedingofone-time.VirulentGooseparvovirusantigenwasusedtoimmunizedgoatsintravenousinjection.High-immunizedserumwith1:8Agarprecipitationantibodytiterswasgetted.Willbepreparedbytheanti-serumandthemarketblastwerecomp
