不同价态无机砷染毒大鼠尿液砷形态代谢产物构成比分析研究

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1、不同价态无机砷染毒大鼠尿液砷形态代谢产物构成比分析研究【摘要】目的通过不同价态染砷大鼠尿液中砷形态含量及比例的分析，寻找不同价态砷代谢和转轨间的差异，为进一步探讨砷代谢产物与砷中毒作用机制间的关系提供基础数据。方法70只大鼠被随机分为7组，即对照组;亚砷酸钠高、中、低剂量组;砷酸钠高、中、低剂量组，70只大鼠分别经口摄入去离子水、亚砷酸钠和砷酸钠1个月后，采集各组大鼠尿样。采用高效液相色谱氢化物发生原子荧光光谱法测定各组染砷大鼠尿液中砷形态的含量，并比较不同价态染砷组的构成比。结果中剂量组，亚砷酸钠染毒大鼠尿液中iAs3

2、+∶iAs5+∶DMA为36.1%∶3.0%∶60.8%;而砷酸钠染毒大鼠尿液中iAs3+∶iAs5+∶DMA为55.9%∶0.8%∶43.4%;两者差异有统计学意义(χ2=8.463，P<0.05)。高、低剂量组比较差异无统计学意义。结论不同价态无机砷的体内代谢和转轨不完全相同。【关键词】高效液相色谱氢化物发生原子荧光光谱法;尿液;砷中毒;砷形态分析　ConstituentratioofmetabolicproductofsodiumarseniteandsodiumarsenateinurineofratWUJun,

3、WUShunhua,JIANGPing,etal(SchoolofPublicHealth,XinjiangMedicalUniversity,Urumqi830011,China)Abstract:ObjectiveToanalyzearsenicSpeciationandconcentrationsinurineofratsindifferentgroups,toseekfordifferentaspectsofmetabolismbetweensodiumarsenateandsodiumarsenite,andt

4、ounderstandfurtherthebasicdataaboutrelationshipbetweenit’smetabolismandmechanismoftoxicity.MethodsSeventyratswererandomlydividedinto7groupssuchascontrolgroup,sodiumarsenitehigh,middleandlowdosegroupandsodiumarsenatehigh,middleandlowdosegroup.When70ratwerefed

5、withoraladministrationrespectivelydeionizedwater,sodiumarseniteandsodiumarsenatefor1months,theurineofeverygroupwerecollected.Thenappiedthehighefficiencyliquidchromatographyandhybridgenesisatomicfluorescencespectroscopy(HPLCHGAFS)todetermineSpeciationandconcentrat

6、ionsofsodiumarsenateandsodiumarseniteandit’sproductofmetabolisminurineofrats.Meanwhile,theconstituentratioindifferentgroupswouldbecompared.ResultsTheconstituentratioofiAs3+,iAs5+andDMAwere36.1%∶3.0%∶60.8%inurinesofratinsodiumarsenitemiddlegroups,whilethecons

7、tituentratioofiAs3+,iAs5+andDMAwere55.9%∶0.8%∶43.4%inurineofratsinsodiumarsenatemiddlegroups,thereweresignificantdifferencesinconstituentratiobetweensodiumarsenateandsodiumarsenite(χ2=8.463，P<0.05).Forhighandlowgroups,thesignificantdifferentinconstituentratiow

8、eredisappeared.ConclusionTheinternalmetabolismissignificantlydifferentbetweensodiumarsenateandsodiumarseniteinurine.Keywords:highefficiencyliquidchromat