资源描述：
《cyclind1、cdk4和rb在大肠癌中的表达及其意义》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、cyclinD1、CDK4和Rb在大肠癌中的表达及其意义?428?肿瘤防治研究2006年第33卷第6期cyclinD1,CDK4和Rb在大肠癌中的表达及其意义陈小贺,孟文格,孟繁杰,谢绍建,李保东,付泽娴,蔡建辉ExpressionandSignificanceofcyclinDl,CDK4andRbintheColorectalCarcinomaCI-IEN~iao-he,MENGWen-ge,MENGFan-jie,XIEShao-jian,LIBao-dong,FUZe-xian,CAIJian-hui
2、DepartmentofGeneralSurgery,TheSecondHospitalofHebeiMedicalUniversity,Shijiazhuang050000.CinaCorrespondingAuthor:CAIJiart-huiAbstract:ObjectiveToinvestigatetherelationshipbetweentheexpressionofcyclinD1,CDK4andRbandclinica1pathologica1characteristicsincolorec
3、ta1carcinoma.MethodscyclinD1,CDK4andRbweredetectedin70colorecta1carcinoma,tumor-adjacentcolorecta1carcinomatissuesandnorma1tissuesbyim—munohistochemica1method.ResultsOverexpressionofcyclinD1andCDK4revealeelin36/70samples(51.4)and28/70samples(40.0)respective
4、lywhichshowedanegativecorrelationwithtumordif—ferentiation(P<0.01).TheexpressionpercentageofcyclinD1andCDK4was70.0and60.0re—speetivelyinmetastasiscancerwhereasonly(44.0)ofeyelinD1and32.0ofCDK4expressionwereshowninnon-metastaticcancer.ThepositiveratesofRb
5、expressionwere65.7insamplesofcolorecta1carcinomas,and78.6intumor-adjacent85.7innorma1tissuesrespectively.Thereisapositiverela—tionshipbetweencyclinD1andCDK4(P<0.05).ConclusionOverexpressionofcyclinD1andCDK4existincolorecta1carcinomaandshowagoodrelationwi
6、thtumordifferentiationand1ymphnodemetasta—sis.AbnormalityofmanygenesinvolvedintheregulatorypathwayofcyclinD1,CDK4andRbmaybein—volvedinthemolecularmechanismofcolorecta1carcinogenesis.Keywords:Colorectalcarcinoma;cyclinD1;cyclindependentkinase4;RbpmteimImmuno
7、histoch~-nistry摘要:目的探讨细胞周期调控因子在大肠癌中的表达及其与大肠癌临床病理特征的关系.方法应用免疫组化SP法对70例大肠癌组织及距癌灶3cm以外的癌旁组织,lOcm以外的正常组织中cy—clinD1,CDK4和Rb进行检测.结果cyclinD1和CDK4在大肠癌中过度表达,分别为36/70(51.4)和28/70(40.0),并与肿瘤的分化程度呈反比,有淋巴结转移的大肠癌,其cyclinD1和CDK4的阳性率分别为70.0和60.0,无淋巴结转移的大肠癌阳性率分别为44.0和32.0,两
8、者相比差异有显着性(P<O.05).Rb在大肠癌,癌旁及正常组织中的表达分别为65.7,78.6和85.7.正常组织与癌组织中Rb的阳性率差异有显着性(P<O.01).cyclinD1与CDK4呈正相关关系(P<O.05).结论cy—clinD1,CDK4的过度表达与肿瘤的分化程度,淋巴结转移密切相关;大肠癌的发生机制涉及cyclinD1,CDK4和Rb调节环路中多个基因的异常.关
