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1、ThePhotodestructionofVirusinNano-TiO2Suspension422DOIl0.1007/sll595.006.3422.6Vo1.22No.3XURuifenetal:ThePhotodestructionofHrusinNano-TiOeSuspensionThePhotodestruetionofVirusinNano-TiO2SuspensionxuRuifen,LIUXil[11)l~!,zHANGP蛐g2,MAHaoLIUG蛐g2,XIAZhengyan(1?CollegeofMaterialsScience
2、andEngineering,BeijingUniversityofChemicalTechnology,Beijing100029.China;2.CollegeofLifeScienceandTechnology,BeijingUniversityofChemicalTechnology,Beijing100029,China;3.NingboGreen.EnjoyNanoTechnologyR&DCOLTD.,Ningbo315300,China)Abstract:HepatitisBsurfaceantigen(HBsAg)wassel
3、ectedasreferencetoevaluatethephotodestructiveeffectofaself-preparednano—TiO,onvirusesinaqueoussuspensionthroughsandwichELISAassay(aninvitroenzymeimmunoassay)underdifferentconditions,andmoregeneralexperimentsonRNA(ribonucleicacid)andcaseinwerecarriedout.ResultsindicatethatTiO.isd
4、estructiveatleasttomostvirusesinwater.Themechanismofdestructionwasdiscussed.Keywords:nano—TiO,;photodestruction;HbsAg;virus1IntroductionTiO,isasafe,stableandemcientsemiconductorphotocatalystunderintensiveresearchforitscapabilityofsolarenergyconversionandthepotentialapplicationsi
5、nsolarcell,watersplitting,environmentalcleanupandetc【".Itsphotodestructionofmicroorganismsisabigbranch.Currently,therearealreadyalotofreportsonthebactericidaleffectofTiO,inaqueoussuspensionssuchasonEscherichiacoli,LactobacillusacidophilusandStaphylococcusaureusJ.anditwasfoundtha
6、tthecomeintocontactwithTiO,andthusdeservespecificinvestigation.SinceHepatitisBvirus(HBV)isarepresentativeinfectiousvirusharassingaboutl/l0ofthepopulationinChina,inthisstudy,weselectedHBsAg,themaincomponentOfHBVcapsid,asthereferencetoiudgethephotodestructionofvirusinnano-TiO,susp
7、ension.andfurtheronamoregeneralbackground.thephotodestructionOfRNAandcaseinwasalsoinvestigated.2Experimentalphotokillingactivitywasassociatedwiththereduction2.1MaterialsinthelevelofintracellularcoenzymeA(CoA)throughphotooxidationthusthecellrespirationwasinhibitedNano-TiO2powder(
8、self-prepared:thesurfacewasmodifiedwithaninorga
