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1、保留指数结合准确质量测定对鱼腥草挥发性成分的定性分析第29卷第2期2010年2月分析测试FENXICESHIXUEBAO(JournalofInstrmnentalAnalysis)VoL29No.2126～130保留指数结合准确质量测定对鱼腥草挥发性成分的定性分析刘素红,王呈仲,苏越,郭寅龙(1.上海中医药大学中医复杂系统研究中心,上海201203;2.中国科学院上海有机化学研究所上海质谱中心,上海200032)摘要:采用顶空一气相色谱一四极质谱法和顶空一气相色谱一飞行时间质谱法对鱼腥草挥发性成分进行检测,运用保留指
2、数结合准确质量测定,分别从鱼腥草茎和叶中鉴定出49,39种挥发性组分,并采用峰面积归一化法测定各组分的相对含量.鱼腥草茎和叶中的挥发性成分差别较大.鱼腥草茎以Ot一蒎烯(12.24%),一蒎烯(22.42%),月桂烯(11.98%),乙酸冰片酯(10.97%),甲基正壬酮(10.83%)为主要成分,而鱼腥草叶则以月桂烯(39.48%),(z)一罗勒烯(26.52%),癸醛(10.35%),桧烯(4.43%),甲基正壬酮(2.44%)为主.结合保留指数和准确质量测定能显着提高质谱定性的准确性,有利于解决质谱检索时定性不确
3、定的问题.关键词:保留指数;准确质量测定;鱼腥草;挥发性成分;GC—MS中图分类号:0657.71文献标识码:A文章编号:1004—4957(2010)02—0126—06doi:10.3969/j.issn.1004—4957.2010.02.005QualitativeAnalysisofVolatileCompoundsfromHouttuyniacordataThunbbyRetentionIndexandAccurateMassMeasurementLIUSu.hong,WANGCheng.zhong,SUY
4、ue,GUOYin.1ong(1.ResearchCenterforTraditionalChineseMedicineComplexitySystem,ShanghaiUniversityofTraditionalChineseMedicine,Shanghai201203,China;2.ShanghaiMassSpectrometryCenter,ShanghaiInstituteofOrganicChemistry,ChineseAcademyofSciences,Shanghai200032,China)Ab
5、stract:Astaticheadspace—gaschromatography—quadrupolemassspectrometry(HS—GC—QMS)andstaticheadspace—gaschromatography—timeofflightmassspectrometry(HS—GC—TOFMS)havebeenutilizedtoanalyzevolatilecompoundsfromfleshHouttuyniacordataThunb.49and39volatilecompoundswereide
6、ntifiedinthestemsandleavesofHouttuyniacordataThunb,respec-tively,bycombinationofretentionindexandaccuratemassmeasurement.Therelativecontentofeachconstituentwasdeterminedbyareanormalization.Amongthesecompounds,34volatilecorn—poundswerefoundbothinthestemsandleaves
7、.TheresultsshowedthatdifferentpartofHouttuyniacordataThunbhaddifferentchemicalcompoundsandtheirrelativecontentswerealsodifferent.OZ—pi—nene(12.24%),-pinene(22.42%),myrcene(11.98%),hornylacetate(10.97%)and2-un—decanone(10.83%)werefoundtobethemajorconstituentsinth
8、estems,whilemyrcene(39.48%),(Z)一ocimene(26.52%),decanal(10.35%),sabinene(4.43%)and2一undecan.one(2.44%)werethemajorcomponentsintheleaves.Theresultindicatedthatthecombi
