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1、TranscriptionActivityofEctogenicHumanCarcinoembryonicAntigenPromoterinLungAdenocarcinomaCellsA549J华ouri中rla"o大ng学Unv学ert报ySre医ne学aT(e英hn德ogy文[版Scj]26㈣:517-519,20o6华中科技大学[医学(英德文)版j…517TranscriptionActivityofEctogenicHumanCarcinoembryonicAntigenPromoterinLungAdenocarcinomaCellsA549
2、'XIONGWeining(熊维宁),FANGHuijuan(方慧娟)#,XUYongjian(徐永健),XIONGShendao(熊盛道),CAOYong(曹勇),SONGQingfeng(宋青凤),ZENGDaxiong(曾大雄),ZHANGHuilan(张惠兰)DepartmentofRespiratoryMedicine,TongjiHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnology,Wuban43003(f肋Summary:Thetranscription
3、activityofectogenichumancarcinoembryonicantigen(CEA)promoterinlungadenocarcinomacellsA549wasinvestigatedforthefurthergene—targetingtherapy.There—portergenegreenfluorescentprotein(GFP)drivenbyCEApromoterandhumancytomegalovirusfCMV1promoterwererelativelyconstructedandnamedplasmidpC
4、EA-EGFPandpCMV—GFPre—spectively.Theintensityoffluorescencewasdetectedbyfluorescencemicroscopeandflowcytome—tryanalysisafterthepCEA-GFPandpSNAV—GFPplasrnidsweretransfectedintoA549cellsthroughliposomerespectively.Theresultsshowed(4.08--.0.63)%oftheA549cellstransfectedwithpCEA-AFPpl
5、asmidexpressed.significantlylowerthanthatoftheA549cellstransfectedwithpCMV—GFP【(43.27_+3.54)%].ItwassuggestedthatectogenichumanCEApromoterinlungadeno-carcinomacellsA549wasweaklyexpressed.ThedistinctspecificityofCEApromoterinCEAhighexpressioncellswasregardedasatoolinselectivegenet
6、herapy.butthetranscriptionactivityofec—togenichumanCEApromoterwasneededtoincreaseinthefuture.Keywords:tissue—specificpromoter;carcinoembryonicantigen;lungcarcinomaDoIl0.1007Isll596—006—0507—6CarcinoembryonicantigenfCEA)isoneofthemostearlydiscoveredandidentifiedtumorassociatedanti
7、gens.Ithashighexpressioninsomeglandulartumors,suchaslungcancer,colorectalcancer,breastcancerandstomachcancer.SomeresearchesdetectedtheactivityofCEApromoterinvarioustumorcellsandfounditcouldspe—cificallypromotetheexogenousgeneexpressioninCEApositivetumorcells,suggestingCEApromoter
8、canbeatargetpromoterintumorgenetherapy.I
