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1、SPF鸡人工感染禽源大肠杆菌、H9亚型禽流感病毒后的体液免疫应答和死亡率分析畜牧兽医,2006,37(9),899~902ActaVeterinariaetZootechnicaSinicaSPF鸡人工感染禽源大肠杆菌,H9亚型禽流感病毒后的体液免疫应答和死亡率分析高璐,胡仁莉,陈祥,盛瑜,刘静,高崧,刘秀梵(扬州大学农业部畜禽传染病学重点开放实验室,扬州225009)摘要:SPF鸡经不同顺序,不同时间间隔人工感染MPAIV和E.coli173株(04)后,对试验鸡的病死率和针对大肠杆菌不同抗原体液免疫应答水平等进行研究.结果表明:各混合感染组试验鸡病死率明显高于单独接种组,且以先接种MPAI
2、V再接种E.coli组死亡率最高;各混合感染组试验鸡针对E.coliOMPs和LPS的抗体水平低于单独感染E.coli组,其中又以先接种MPAIV再接种E.coli组为最低,提示MPAIV和E.coli间存在协同致病作用,这种协同机理可能与因MPAIV的感染导致一定程度的免疫抑制并进而促进了E.coli在体内的定居与繁殖有关.关键词:SPF鸡;大肠杆菌;低致病性禽流感病毒;协同致病;免疫抑制中图分类号:$858.315.3文献标识码:A文章编号:0366—6964(2006)09—0899—04TheMortalityRateandImmuneEfficacyIntratracheallywi
3、thLowlyVirulentE.ofChickensInoculatedcolland/orMPAIVGAOLu,HURen—li,CHENXiang,SHENGYu,LIUJing,GAOSong,LIUXiu—fan(AnimalInfectiousDiseaseLaboratory,MinistryofAgriculture,Yangzhou225009,China)Abstract:10一day—oldspecificpathogenfree(SPF)chickenswereinoculatedintratracheallywithlowlyvirulentE.coliand/orm
4、ildlypathogenicavianinfluenzavirus(MPAIV).Theresultswererevealedasfollows:(1)ThemortalityrateofchickensinoculatedwithE.colandMPAIVsimultaneouslyperbirdwas33.3.ChickensinoculatedfirstwithMPAIVandthenE.coliatanintervalof48hoursexperiencedthemortalityrateof66.79/5,whereasthoseinoculatedwithE.coliandthe
5、nMPAIVatthesameintervalexhibitedthemortalityrateof16.7,thegroupinfectedwithMPAIValoneexperiencedthemortalityrateof11.1whilethegroupinfectedwithE.colialonewasfoundnodeath.TheseresultssuggestedthatMPAIVmightlongthentheperiodsthatE.colicolonizedinthetracheaandlungofinoculatedchickens,anditmightfacili—t
6、ateE.coliinvasionintotherespiratorytractofthechickensresultinginmoreseverepathologicabnormalities.TherewasapathogenicsynergismbetweenE.coliandMPAIV.(2)Anindirectenzyme—linkedimmunosorbantassayfordetectionofantibodiestoOMPsandanindirecthemag—glutinationassayforLPSofpathogenicE.colifromchickenoriginwe
7、reusedrespectively.TheantibodiestoOMPsandLPSofthebirdsinoculatedfirstwithMPAIVandthenE.coliwerelOW—erthanthoseofothergroups.ThisresultsindicatedthatthesynergisticpathopoiesisbetweenE.∞liandMPAIVseemed
