组织培养主要培养基的种类与组成

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1、主要培养基的种类与组成一.White培养基二、LS培养基RM-64三.HB培养基四．MS培养基RM—62五.花肥培养基六．KC培养基七.Kassanis培养基八.木本植物用培养基九.Nitsch&Nitsch(1969)培养基十.Nielsen(1960)培养基十一.MT培养基十二.Miller培养基(1963)十三.B5培养基十四.N6培养基十五.H培养基十六.改良White培养基  一.White培养基,White(1963)1.无机盐类Ca(NO3)2·4H2O287mg/LKNO380mg/LKCl65mg/L

2、NaH2PO4·H2O19.1mg/LMgSO4·7H2O738mg/LNa2SO4：·10H2O453mg/LMnSO4·4H2O6.6mg/LH3BO31.5mg/LZnSO4·7H2O2.7mg/LKI0.75mg/L2．有机物甘氨酸3.0mg/L烟酸0.5mg/L维生素B60.1mg/L维生素B10.1mg/L柠檬酸2.0mg/L蔗糖20g/LpH5．7二、LS培养基RM-64[Linsmair&SKoog(1964))]1．无机盐NH4N03l650mg／LKNO31900mg/LCaCl2·2H2O440m

3、g/LMgSO4·7H2O370mg/LKH2PO4170mg/LNa2一EDTA37.3mg/LFeSO4·7H2O27.8mg/LH3BO36.2mg/LMnSO4·4H2O22.3mg/LZnSO4·7H2O8.6mg/LKI0.83mg/LNa2MoO·2H2O0.25mg/LCuSO4·5H2O0.025mg/LCoCl2·6H2O0.025mg/L2．有机物维生素Bl0．4mg/L肌醇10．0mg／L蔗糖30g／LpH5．6*FeSO4·7H2O5．57g，Na2一EDTA7．45g先溶于1升蒸馏水里，然后

4、按每升培养基含5mg的比例加入培养基中。三.HB培养基Holley&Baker(1963)1.无机盐(以1/2浓度的Knop液为基础)Ca(NO3)2.4H2O1000mg/LKNO3125mg/LMgSO4·7H2O125mg/LKH2PO4125mg/LBerthelot's液0.5ml/LBerthelot's液成分组成:MnSO4·4H2O20g/LKI0.5g/LNiCl2·6H200.05g/LCoCl2·6H200.05g/LZnSO4·7H200.10g/LCuSO4·5H2O0.05g/LBeSO4·

5、4H2O0.10g/LH3BO30.05g/L浓硫酸1ml2.有机物维生素B1理学1mg/L腺嘌呤8mg/LNAA2mg/L蔗糖40g／L四．MS培养基RM—62(Murashige&Skoog(1962))1.无机盐类同LS培养基2．有机物 五.花肥培养基Kano(1963)(Kyotoso1ution)花肥3g/LKH2PO4250mg/LCa(NO3)2.4H2O1000mg/LMgSO4·7H2O250mg/L(NH4)2SO42mg/L蔗糖20g／LpH5.0六．KC培养基KnudsonC(1964)1.无机

6、盐KH2PO4250mg/LCa(NO3)2.4H2O1000mg/L(NH4)2SO4500mg/LMgSO4·7H2O250mg/LFeSO4·7H2O25mg/LMnSO4·4H2O7.5mg/L2.有机物蔗糖20g／LpH5.0～5.2七.Kassanis培养基(1957)1.无机盐Ca(NO3)2.4H2O500mg/LKNO3125mg/LMgSO4·7H2O125mg/LKH2PO4125mg/LBerthelot's液10滴2.有机物酪蛋白1mg/L半胱氨酸10mg/L腺嘌呤5mg/L烟酸1mg/L维生

7、素B61mg/L泛酸钙10mg/L肌醇0.1mg/L维生素H(生物素)0.01mg/L蔗糖20g／L八.木本植物用培养基(WPM,WoodyPlantmedium)1.无机盐NH4N03400mg／Ca(NO3)2.4H2O556mg/LK2SO4990mg/LCaCl2·2H2O96mg/LKH2PO4170mg/LNa2MoO4·2H2O0.25mg/LMgSO4·7H2O370mg/LMnSO4·H2O22.4mg/LZnSO4·7H2O8.6mg/LCuSO4·5H2O0.25mg/LFeSO4·7H2O27.

8、8mg/LNa2-EDTA37.3mg/L2.有机物肌醇100mg/L维生素B11.0mg/L烟酸0.5mg/L维生素B60.5mg/L甘氨酸2.0mg/L蔗糖20g／L琼脂6g／LpH5.2九.Nitsch&Nitsch(1969)培养基1.无机盐KNO3950mg／LNH4N03720mg／LKH2PO468mg/LCaCl2