主要培养基的种类与组成.doc

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1、主要培养展的种类与组成-.White培养基二、LS培养基RM-64三.HB培养基四.MS培养基RM-62五.花肥培养基六.KC培养基七.Kassanis培养基八.木木柏物用培养基九.Nitsch&Nitsch(1969)培养基l-.Nielsen(1960)培养基

2、—.MT(murashgeandTucker)培养基I二Miller培养基(1963)十三.B5培养基十四.N6培养基十五.H培养基十六.改良White培养基%1.White培养基，White(1963)1.无机盐类Ca(NO3)2.4H2O287

3、mg/LKN0380mg/LKQ65mg/LNaH2PO4?H2O19.1mg/LMgS()4?7H2O738mg/LNa2SO4：?10H2O453mg/LMnSO4?4H2O6.6mg/LH3BO31.5mg/LZnSO4?7H2O2.7mg/LKI0.75mg/L2.有机物tt氨酸3.0mg/L烟酸0.5mg/L维生索B60.1mg/L维生索B10.1mg/L柠檬酸2.0mg/L裁糖20g/LpH5.7二、LS培养基RM-64[Unsmair&SKoog(1964))]1.无机盐NH4N03I650mg

4、/LKNO31900mg/LCaO2?2H2O440mg/LMgS()4?7H2O370mg/LKH2PO4170mg/LNa2一EDTA37.3mg/LFeS04?7H2O27.8mg/LH3BO36.2mg/LMnSO4?4H2O22.3mg/LZnSO4?7H2O8.6mg/LKI0.83mg/LNa2MoO?2H200.25mg/LCuSO4?5H2O0.025mg/LCoO2?6H200.025mg/L1.有机物维生索BIO.4mg/LW?10.Omg/L蔗糖30g/LpH5.6*FeS04?7H2

5、O5.57g,Na2—EDTA7.45g先溶于1升蒸ta水里，然后按每升培养基含5mg的比例加入培养基屮。%1.HB培养基Holley&Baker(1963)1.无机盐(以1/2浓度的Knop液为基础)Ca(N03)2.4H201000mg/LKNO3125mg/LMgS()4?7H2O125mg/LKH2PO4125mg/LBerthelot's液0.5ml/LBerthelot's液成分组成:MnSO474H2O20g/LKI0.5g/LNiO2?6H200.05g/LCoO2?6H200.05g/LZn

6、SO4?7H200.10g/LCuSO4?5H2O0.05g/LBeSO4?4H2O0.10g/LH3BO30.05g/L浓硫酸1ml2.有机物维生索B1理学1mg/L腺嗓吟8mg/LNAA2mg/L蔗糖40g/L%1.MS培养基RM-62(Murashige&Skoog(1962))1.无机盐类同LS培养基2.有机物%1.花肥培养基Kano(1963)(Kyotosolution)花肥3g/LKH2PO4250mg/LCa(NO3)2.4H2O1000mg/LMgS()4?7H2O250mg/L(NH4)2

7、SO42mg/L蔗糖20g/LpH5.0六.KC培养基KnudsonC(1964)1.无机盐KH2PO4250mg/LCa(NO3)2.4H2O1000mg/L(NH4)2SO4500mg/LMgS()4?7H2O250mg/LFeS04?7H2O25mg/LMnSO4?4H2O7.5mg/L2.有机物廉糖20g/LpH5.0〜5.2七.Kassanis培养基(1957)1.无机盐Ca(NO3)2.4H2O500mg/LKNO3125mg/LMgS()4?7H2O125mg/LKH2PO4125mg/LBer

8、thelot's液10滴2.有机物酪蛋口1mg/L半胱氨酸10mg/L腺喋吟5mg/L烟酸1mg/L维生索B61mg/L泛酸钙10mg/L肌醉0.1mg/L维生索H(生物索)0.01mg/L滋糖20gL八.木木植物用培养基(WPM,WoodyPlantmedium)1.无机盐NH4N03400mg/Ca(NO3)2.4H2O556mg/LK2SO4990mg/LCaa2?2H2O96mg/LKH2PO4170mg/LNa2Mo04?2H200.25mg/LMgS()4?7H2O370mg/LMnSO4?H2O

9、22.4mg/LZnSO4?7H2O8.6mg/LCuSO4?5H2O0.25mg/LFeSO4?7H2O27.8mg/LNa2-EDTA37.3mg/L2有机物肌醉100mg/L维生素B11.0mg/L烟酸0.5mg/L维生索B60.5mg/Ltt氨酸2.0mg/L廉糖20g/L琼脂6g/LpH5.2WPM培养基:化学名称中文名称mg/L备注8叫）2・4屯0硝酸钙556大量兀素NH^NOg硝酸镀4