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1、TNFα和VEGF在激素性股骨头坏死中的变化第l4卷第l2期2006年6月中国矫形外科杂志OrthopedicJournalofChinaV01.14.No.12Jun.2OO6TNFot和VEGF在激素性股骨头坏死中的变化△胡志明,王海彬,李祖国,周斌,王小宁(1.南方医科大学分子免疫学研究所,广州510515;2.广州中医药大学第一附属医院骨科,510050)摘要:[目的】通过应用马血清,激素作用新西兰兔,诱导激素性股骨头坏死动物模型.分析TNFct,VEGF在激素性股骨头坏死中的作用.【方法]将20只健康新西兰兔随机分成2组,每组lO只.A组为
2、模型组.每只用马血清10ml/kg.经兔耳缘静脉注射.间隔2周,按5ml/kg剂量连续2d注射马血清各1次.间隔2周后,注射醋酸泼尼松龙.按7.5mg/kg腹腔注射3次.B组:正常对照组.动物注射激素5周后,采血,应用ELISA法测血清中TNF-a浓度.处死模型组,标本进行常规组织病理学检查,免疫组化分析股骨头VEGF的表达.[结果】模型组兔血清TNF-a浓度明显高于正常组(P<0.01).组织病理学检查显示:部分血管栓塞.骨髓腔内造血组织明显减少.免疫组化结果显示:骨细胞及骨髓组织VEGF表达明显减少.[结论]血清中TNF—a浓度升高及股骨头
3、骨髓组织VEGF表达减少可能是激素性股骨头坏死发生的重要因素.关键词:股骨头坏死;肿瘤坏死因子;血管内皮生长因子中图分类号R681.8文献标识码AChangeofTNF?OtandVEGFonglucocorticoid?inducedavascularnecrosisoffemoralheadinrabbits//HUZhi-ming,WANGHai—bin,LIZu—guo.eta1.InstituteofMolecularImmunology.SouthernMedicalUniversity.Guangzhou510515Abstract:[
4、Objective]Toestablishanimalmedelofavascularnecrosisofthefemoralheadinrabbitsinducedbyglucocorti-coid,tofurtherinvestigatethepathogenesisofglucocorticoid?inducedosteonecrosis.[Method]TwentyNewZealandwhiterabbitswereusedandrandomlydividedintotwogroups.GroupAwasinjectedwithhorsese
5、rumandprednisone.GroupBwasuseda8thecontro1.TheconcentrationofTNF-wasmeasul~byELISAinrabbitsserumofgroupAandgroupB.Femoralheadspoci-menswerestudiedbyhistopathologicexaminationandtheexpressionofVEGFbyimmunohistochemicalexamination.[Result]TheconcentrationofTNF一仅inrabbitsserumofgr
6、oupAwashigherthanthatofgroupB(P<O.O1).IngroupA,whichWaSfea-turedwithdefectiveperiosteum.therewereosteocytespyknosisornecrosis.increaSednumbersofemptybonelacunaeandfatcells,decreasedhematopoietictissueandoccludedbloodvesselscomparedtonormalcontrolgroupunderlightmicroscopeandh
7、is-tologicalstaining.TheexpressionofVEGFWasdecreaseintheosteonecrosis.[Conclusion]TherisingoflevelofTNF—andde-ceaseofexpressionofVEGFmaybeoneofthemostimportantfactorsintheoccurrentofavascularnecrosisofthefemoralhead.Keywords:Osteonecrosisoffemoralhead;Tumornecrosisfactor-a(TNF一
8、仅);VaScularendothelialgrowthfactor(VEGF)股骨头缺血性坏死是I临床多发
