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1、中国水稻科学(ChinJRiceSci),２０１４,２８(６):６０５－６１１http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１４．０６．００６６０５水稻紫色果皮的延迟遗传及Pb基因功能标记开发王芳权１,２杨杰１,∗范方军１王军１朱金燕１李文奇１沈文飚２仲维功１(１江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心,南京２１００１４;２南京农业大学生命科学学院,南京２１００９５;∗通讯联系人,EＧmail:yangjie１６８＠aliyun．com)De
2、layedInheritanceofPurplePericarpinRiceandDevelopmentofFunctionalMarkerforPbGeneWANGFangＧquan１,２,YANGJie１,∗,FANFangＧjun１,WANGJun１,ZHUJinＧyan１,LIWenＧqi１,SHENWenＧbiao２,ZHONGWeiＧgong１(１InstituteofFoodCrops,JiangsuAcademyofAgriculturalSciences/JiangsuHighQualityRiceR&
3、DCenter,Nanjing２１００１４,China;２CollegeofLifeScience,NanjingAgriculturalUniversity,Nanjing２１００９５,China;∗Correspondingauthor,EＧmail:yangjie１６８＠aliyunc．om)WANGFangquan,YANGJie,FANFangjun,etal．DelayedinheritanceofpurplepericarpinriceanddevelopmentoffunctionalmarkerforP
4、bgene．ChinJRiceSci,２０１４,２８(６):６０５Ｇ６１１．Abstract:Inordertodeterminetheheredityofpurplepericarp,thepurplepericarpcultivarXiangxuenuo,developedinJiangsuProvince,andwhitepericarpcultivar０２４２８wereusedasparentstomakereciprocalcrosses,andthecolouroftheseedsofrelevantF１,F
5、２,F３generationwerephenotyped．TheresultsindicatedthatthepurplepericarpofXiangxuenuowascontrolledbysingledominantnucleargene,belongingtothetypicalmaternalinfluence．Purplepericarpinricewasduetoa２bpdeletioninPbgeneonchromosome４whichencodedabasichelixＧloopＧhelixprotei
6、n．ACAPSmarkerwasdevelopedbasedonthe２bpdeletion,andtheCAPSmarkerwascoＧsegregatedwiththecolourofpericarpintheF２population,allowingtoidentifythehomozygouspurplepericarpgenotype,homozygouswhitepericarpgenotypeandheterozygouspurplepericarpgenotypeindividuals．ThePCRpro
7、ductsof１０ricelinesweresequenced．AllthepurplepericarpricelinescarriedGTdeletion．Itwasinterestingthatthewildricelineswithredpericarpcarriedthenormalallele;thereforewespeculatedthatthepurplepericarpwasmutatedfromcultivatedrice．Furthermore,onelinewithpurplehullandwhi
8、tepericarpcarriedpballele,itshowedthecomplexgeneticmechanisminricepigmentation．Inconclusion,theCAPSmarkerisaperfectmarkerforpurplepericarpricebreedingandtheevo
