欢迎来到天天文库
浏览记录
ID:58298876
大小:350.01 KB
页数:5页
时间:2020-05-04
《原发性肝癌患者外周血Th22细胞水平的变化及意义-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、许秀华等原发性肝癌患者外周血Th22细胞水平的变化及意义第9期·1245·doi:10.3969/j.issn.1000-484X.2014.09.023原发性肝癌患者外周血Th22细胞水平的变化及意义许秀华向晓星马莉①徐跃元刘国芳(苏北人民医院,扬州大学临床医学院消化内科,扬州225001)中图分类号R735.7文献标志码A文章编号1000-484X(2014)09.1245-05[摘要]目的:探讨Th22细胞在乙肝相关性原发性肝癌(PHC)患者外周血的表达水平及临床意义。方法:收集16例乙肝肝硬化、14例乙肝相关性PHC合并肝硬化患者和l
2、6例健康对照者外周血,荧光实时定量聚合酶链反应(RT.PCR)检测血清乙型肝炎病毒(HBV)DNA载量,流式细胞术检测三组外周血Th22、Thl、Thl7和Treg细胞所占比例,并逐层分析比较。结果:Th22和Thl7细胞在PHC组明显高于肝硬化组和对照组(P<0.05),肝硬化组高于对照组(P>0.05)。Thl细胞在PHC组和肝硬化组明显高于对照组(P<0.05),其中PHC组低于肝硬化组(P>0.05)。Treg细胞PHC组、肝硬化组和对照组依次下降(P<0.05)。肿瘤直径与Th17和Th22细胞呈显著正相关(P<0.05),与Thl
3、和Thl7细胞无显著相关性(P>0.05)。HBVDNA与Thl、Thl7、Th22和Treg细胞均无显著相关性(P>0.05)。结论:Th22细胞可能与Thl、Thl7和Treg细胞一样,均参与PHC的发生和进展,且与乙肝病毒复制情况无关,其作用机制可能与Thl7细胞类似或通过Thl7细胞发挥作用。[关键词]肝肿瘤;肝炎病毒,乙型;Th22细胞;发病机制;CIMT细胞ChangeandsignificanceofTh22cellsinpatients、)l,ithprimaryhepaticcarcinomaxuXiu—Hua,XIANGX
4、iao一,MALi,XUYue—Yuan,LIUGuo—Fn.DepartmentofGastroenterology,NorthernJiangsuPeoplesHospital,ClinicalMedicalCollegeofYangzhouUniversity,Yangzhou225501,China[Abstract]Objective:ToinvestigatetheexpressionofTh22cellsinperipheralbloodofprima~hepaticcarcinomapatientsinfectedwithhe
5、patitisBvirus(HBV)anditsclinicalsignificance.Methods:Peripheralbloodof16patientswithlivercirrhosis,14patientswithcirhoticpfima~hepaticcarcinomaand16healthycontrols,allofpatientswereinfectedwithHBV.SerumHBVDNAlevelsweremeasuredbyfluorescencereal—timequantitativepolymerasecha
6、inreaction(RT-PCR),andthepercentagesofTh22,Thl,Thl7andTregcellswereassessedbyflowcytometry(FCM).Results:ThepercentagesofThl7andTh22cellsinPHCpatientsweresignificantlyhigherthanthatinlivercirrhosispatientsandhealthycontrols(P<0.05),andThepercentagesofThlcellsinPHCpatientsand
7、livercirrhosisweresignificantlyhigherthanthatinhealthycontrols(P<0.05),andthelevelinPHCpatientswerelowerthanthatinlivercirrhosispatients(P>0.05).ThepercentagesofTregcellsweredecreasedsuccessivelyinPHCpatients,livercirhosispatientsandhealthycontrols(P<0.05).InPHCpatients,The
8、tumordiameterwaspositivelycorelatedsignificantlywiththepercentagesofThl7andTh22cel
此文档下载收益归作者所有