大豆种质资源叶型和荚粒性状的关系及与SSR标记的关联分析-论文.pdf

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1、作物学报ACTAAGRONOMICASINICA2012，38(7)：1196—1204http：／／www．chinacrops．org／zwxb／ISSN0496—3490；CODENTSHPA9E—mail：xbzw@chinajournat，net。cnDoI：10．3724／SEJ．1006．2012．01196大豆种质资源叶型和荚粒性状的关系及与SSR标记的关联分析伍宝朵陈海峰郭丹丹沙爱华单志慧张晓娟杨中路邱德珍陈水莲朱晓玲张婵娟周蓉周新安中国农业科学院油料作物研究所／农业部油料作物生物学重点开放实验室，湖北武汉430062摘要

2、：选用167份国内外大豆品种资源材料，依据叶长／宽比值将其划分为宽叶型和窄叶型，分析叶型相关性状与荚粒性状的相关性，结果表明叶宽、叶长／宽比均与每荚粒数显著相关(r=一0．69和0．64，P<0．001)。选用均匀分布于大豆20条连锁群上的65个SSR标记分析资源材料的群体结构，并用目标区间内l3个SSR标记与叶型相关性状和荚粒性状进行关联分析，结果显示，标记20．285与每荚粒数显著关联，推测控制每荚粒数的基因可能位于标记20—285附近；标记20—26和20．45间的标记几乎都与叶长／宽比存在显著的关联性，推测控制叶型的基因位于标记20

3、—26和20．45之间。相关分析和关联分析证实大豆叶型与荚粒性状存在密切关系，并缩小了每荚粒数和叶型候选基因的区间。关键词：大豆；叶型；荚粒性状；相关性分析；关联分析RelationshipofLeafletShape，PodTraitsandAssociationwithSSRMarkersinSoybeanGermplasmWUBao—Duo，CHENHai．Feng，GUODan—Dan，SHAAi—Hua，SHANZhi—Hui，ZHANGXiao—Juan，YANGZhong．Lu，QIuDe—Zhen，CHENShui—Lian

4、，ZHUXiao—Ling，ZHANGChan．Juan，ZHOURong，andZHOUXin—AnOilCropsResearchInstituteofChineseAcademyofAgriculturalSciences／KeyLaboratoryofOilCropBiology，MinistryofAgriculture，Wuhan430062，ChinaAbstract：Podtraitsareimportantfactorsafectingsoybeanyield，and血evhavebeenidentifiedtotight

5、lyassociatewithleafletshape．Inpresentstudy,leafletshape／podtraitswereanalyzedusing167soybearlaccessions．whichwereclassifiedintotwotypes(broadandnarrowleaflets)basedontheratioofleaflength／width．Correlationanalysiswascarriedoutbetweenleafletshapere—latedtraitsandpodtraits：AN

6、0VAanalysiswasalsoperformedusing65SSRmarkers，evenlydistributedin20linkagegroups；associationanalysiswascarriedoutusing13SSRmarkersonthetargetregions．Marker20—285，locatingonchromosomeI，highlyassociatedwithnumberofseedsperpod．indicatingthatthegenecontrollingnumberofseedsperpo

7、dmayco—locatewimmarker20—285．Almostallmarkersbetween20—26and20—45wereassociatedwithratio0fleaflength／width．wespeculatedthatthegenescontrollingleafletshapewerelocatedintheregionfrommarker20．26t020—45．Hence．itwasprovedthetargetregionharboringcandidategenesofleafletshapeandpo

8、dnumberwasnarrowed．Ourstudymayprovideapotentialapproachinmolecu—larmarker-assistedbreedin