间充质干细胞体内成骨的实验研究（医学论文）.doc

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1、间充质干细胞体内成骨的实验研究作者：李章华，彭昊，廖文，张玉富，赵强，王常勇【关键词】组织工程化骨摘要：［目的］探讨间充质干细胞在体内的成骨能力。［方法］从羊骼山脊处抽取10~15ml骨髓组织，用Percoll分离液按梯度离心法分离出间充质干细胞，培养、增殖后种植在多孔B磷酸三钙上，构建组织工程化骨，植入8只羊的左后肢跖骨缺损区（长2lmm）作为实验组；单纯植入多孔P磷酸三钙陶瓷材料的8只羊作为对照组;分别在术后6、12、24周处死动物行放射学、组织学和生物力学检测。［结果］放射学和组织学检测，术后6周实验组即可见有新骨生成，对照组则无明显新骨生成；骨缺损部位新生骨样组织、编织骨和板状骨出现

2、的时间实验组也都较对照组早，并且不经软骨介导即能直接成骨，而对照组从两端以“爬行替代”方式成骨。术后24周，放射学和生物力学检测显示实验组骨缺损几乎完全修复，对照组只有部分愈合。［结论］间充质干细胞不仅在体外具有良好的增殖能力，回植入体内后仍然具有良好的成骨能力,不需经过软骨过程就能直接形成新骨，显示了间充质干细胞作为骨组织工程种子细胞良好的应用前关键词：组织工程化骨；骨缺损；新生骨；间充质干细胞Abstract：[Objective]Toinvestigatetheosteogenicabilityinvivoofmesenchymalstemcells(MSCs)[MethodJTenf

3、ifteenmlbonemarrowaspirateswereharvestedfromtheiliaccrestofsheepandenrichedforMSCsbydensitygradientcentrifugationoveraPercollcushion(1073g/ml)Afterculturedandproliferated?thetissueengineeredboneswereconstructedwiththesecellsandseededontoporous13tricalciumphosphateceramics(13TCP)Then,theconstructswe

4、reimplantedintoleftmetatarsusdefect(21mminlength)of8sheepasanexperimentalgroupPorouspTCPcomposedw让hbonemarrowwasimplantedintodefectsofsamesizeandpositionin8sheepasacontrolgroupSheepweresacrificedinthe6th?12th,and24thweekpostoperatively?andtheimplantedsampleswereexaminedbyradiograph,histology,andbio

5、mechanicalanalysis[Result]Newbonetissuewasobservedeitherradiographicallyorhistologicallyatthedefectareaofexperimentalgroupasearlyasthe6thweekpostoperatively;butwasnotobservedinthecontrolgroupBecauseofthenewboneformedinadirectmannerwithoutprogressionthroughacartilaginousprocessintermediately?osteoid

6、tissue,wovenboneandlamellarbone,occurredearlierintheexperimentalgroupthaninthecontrolgroup,inwhichthebonedefectswererepairedinaucreepsubstitution,,mannerAtthe24thweek,radiographsandbiomechanicaltestsrevealedanalmostcompleterepairofthedefectinexperimentalgroup,butonlyapartialrepairinthecontrolgroup[

7、Conclusion]MSCshavegoodreproductiveactivitynotonlyinvitro,butalsoinvivoThenewboneformedinadirectmannerwithoutprogressionthroughacartilaginousprocessintermediatelywhenMSCsweretransplantedinvivoTheresultsdemo