水稻的侧根发育及其影响因素.pdf

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1、作物学报ACTAAGRONOMICAS1NICA2014，4O(8)：1403—1411http：／／zwxb．chinacrops．org／ISSN0496—3490；CODENTSHPA9E—mail：xbzw@chinajouma1．net．caDoI：10．3724／SP．J．1006．2014．01403水稻的侧根发育及其影响因素刘大同荆彦平陈晶晶余徐润王忠扬州大学生物科学与技术学院／江苏省作物遗传生理重点实验室，江苏扬州225009摘要：为了探明水稻侧根发育的过程和影响因素，以日本晴(

2、粳稻)、扬稻6号(籼稻)的不定根和种子根为材料，观察了侧根发育过程中的形态和细胞结构的变化，研究了生长调节物质、光照等因子对侧根发育的影响。结果显示，水稻侧根的发生起始于中柱鞘。相邻的内皮层细胞参与了侧根原基的发生和生长。侧根原基通过顶端生长点的分裂、分化和基部细胞伸长向外生长，侧根在露出过程中改变了母根皮层和表皮结构。外源生长素可促进侧根的发生，但浓度过高则抑制侧根原基的露出；稻苗切除芽后侧根发生减少，主根生长量下降；切除根尖打破了主根顶端优势，促进侧根发生和生长。单侧照光能诱导向光侧的侧根发生

3、。脱落酸在低浓度下对侧根发生有促进作用；Ca促进侧根发生和主根生长，而EDTA抑制侧根发生和主根生长。种子根和不定根的生长对试验因子的响应趋势基本一致。关键词：水稻；侧根发育；中柱鞘；内皮层；生长素；光照RiceLateralRootDevelopmentandItsImpactFactorsLIUDa—Tong，JINGYan．Ping，CHENJing．Jing，YUXu．Run，andWANGZhongCollegeofBioscienceandBiotechnology，YangzhouU

4、niversity／KeyLaboratoryofCropGeneticsandPhysiologyofJiangsuProvince，Yangzhou225009，ChinaAbstract：TheadventitiousrootsandprimaryrootsofNipponbareandYangdao6wereusedtomeasurelateralrootnumberandthegrowthincrementbyImageJsoftwarewithscanningphotograph．Mo

5、rphologicalandstructuralchangesoflateralrootprimo—diawereobservedbyapplyingSpurrresinembeddingandsemi—thinsectioning，andlightmicroscopy．Impactsofthelateralrootemergenceontheparentrootstructurewasobservedbyfluorescencemicroscopyandscanningelectronmicro

6、scopy．1'reatmentswithplanthormones．metalions．illumination，andcuttingoffshootsorroottipsweresetuptoanalysetheirinfluenceonlateralrootdevelopment．Resultsshowedthatricelateralrootinitiationoccu~edfromthepericycle．Theendodermisalsopagicipatedinthelateralr

7、ootprimodiaformationandplayedprotectiveaswellasassistantrolesintheprocess．Theoutwardgrowthoflateralrootprimordiawasrealizedbycelldivisionsofapicalmeristemandcellelongationatthebasearea．Thecodexandepidermisstructuresofparentrootswerealsoalteredduringth

8、eprocessoflateralrootemergence．ExogenousIAApromotedtheoccurrenceoflateralroots．butitshighconcentrationsinhibitedlateralrootemergence．Thenumberoflateralrootsandgrowthofprimaryrootsweresignificantlydecreasedwhenshootswerecutof．Theapica1dominance