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时间:2020-05-23
《黄连解毒汤对Aβ25-35诱导AD大鼠学习记忆能力及相关蛋白激酶表达的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、·1416·中华中医药杂志(原中国医药学报)2014年5月第29卷第5期CJTCMP,May2014,Vo1.29,No.5论著·黄连解毒汤对AI325诱导AD大鼠学习记忆能力及相关蛋白激酶表达的影响伍文彬,李斌,王飞,王永炎(成都中医药大学,成都610075;中国中医科学院,北京100700)摘要:目的:探讨黄连解毒汤(HJD)对A13诱导AD大鼠学习记忆能力和相关蛋白激酶表达的影响。方法:SD雄性大鼠72只,随机分为假手术组、模型组、阳性药多奈哌齐组、黄连解毒汤高、中、低剂量组(6、3、1.5g
2、/kg),每组12只。除假手术组外,在大鼠双侧海马区立体定向注射Ap诱导AD大鼠模型,假手术组定向注射0.9%氯化钠溶液。术后连续灌胃30d,进行Morris水迷宫实验,测试结束后处死动物,摘全脑采用免疫组化和蛋白印迹法检测皮层相关蛋白激酶GSK.3B和CDK一5的表达。结果:黄连解毒汤可缩短AD大鼠平均逃避潜伏期(P<0.05,P3、P<0.05,P<0.01)。结论:黄连解毒汤能够改善AB诱导AD模型大鼠的学习记忆能力,具有较好的益智作用,其机制可能与抑制脑内tau蛋白磷酸化关键蛋白激酶GSK.3B及CDK一5的活性,进而调控tau蛋白磷酸化水平,从而减少N兀在细胞内聚积有关。关键词:黄连解毒汤;阿尔茨海默病;糖原合成酶激酶一3B;周期蛋白依赖性激酶一5基金资助:中国博士后科学基金面上项目(No.2011M501399),中国博士后科学基金特别资助(No.2012T50768),国家自然科学基金青年基金项目(No.8l30324、83),四川省科技创新苗子工程资助项目(No.20132054)EffectsofHuanglianJieduDecoctiononlearningandmemorizingabilityandexpressionoftherelatedproteinkinaseinADratsinducedbyAp25.35WUWen—bin,LIBin,WANGFei,WANGYong.yan(ChengduUniversityofTCM,Chengdu610075,China;ChinaAcademyofCh5、ineseMeScalSciences,Beijing100700,China)Abstract:Objective:ToinvestigatetheeffectsofHuanglianJieduDecoction(HJD)onlearningandmemorizingabilityandexpressionoftherelatedproteinkinaseinADratsinducedbyAp25m.Methods:72maleSDratswererandomlydividedintosixgro6、ups:asham—operationgroup,amodelgroup,donepezilgroup,HJDatahighdosegroup(6g/kg),HJDatamediumdosegroup(3g/kg)andHJDatalowdosegroup(1.5g/kg),with12ratsineachgroup.Theratsinsham—operatedgroupwereinjectedwithnormalsaline(NS),andtheratsinothergroupswereinjec7、tedwithAp25—35inhippocampus.Theratsweregivencorrespondingdrugbygavageonceadayfor30daysaftersurgery.Therats’learningandmemorizingabilitywereevaluatedbytheMorriswatermaze.ThentheyweresacrificedandtheirbrainsweretakenfordeterminationofthecorticalGSK一3Band8、CDK一5expressionbyimmunohistochemistryandwesternblotting.Results:HuanglianJieduDecoctiondecreasedthemeanescapelatencyindirectionalnavigationtest(P<0.05,P<0.01),increasedthetimesoftraversingplatform(P<0.05),andincreasedtheeffectivequadran
3、P<0.05,P<0.01)。结论:黄连解毒汤能够改善AB诱导AD模型大鼠的学习记忆能力,具有较好的益智作用,其机制可能与抑制脑内tau蛋白磷酸化关键蛋白激酶GSK.3B及CDK一5的活性,进而调控tau蛋白磷酸化水平,从而减少N兀在细胞内聚积有关。关键词:黄连解毒汤;阿尔茨海默病;糖原合成酶激酶一3B;周期蛋白依赖性激酶一5基金资助:中国博士后科学基金面上项目(No.2011M501399),中国博士后科学基金特别资助(No.2012T50768),国家自然科学基金青年基金项目(No.8l3032
4、83),四川省科技创新苗子工程资助项目(No.20132054)EffectsofHuanglianJieduDecoctiononlearningandmemorizingabilityandexpressionoftherelatedproteinkinaseinADratsinducedbyAp25.35WUWen—bin,LIBin,WANGFei,WANGYong.yan(ChengduUniversityofTCM,Chengdu610075,China;ChinaAcademyofCh
5、ineseMeScalSciences,Beijing100700,China)Abstract:Objective:ToinvestigatetheeffectsofHuanglianJieduDecoction(HJD)onlearningandmemorizingabilityandexpressionoftherelatedproteinkinaseinADratsinducedbyAp25m.Methods:72maleSDratswererandomlydividedintosixgro
6、ups:asham—operationgroup,amodelgroup,donepezilgroup,HJDatahighdosegroup(6g/kg),HJDatamediumdosegroup(3g/kg)andHJDatalowdosegroup(1.5g/kg),with12ratsineachgroup.Theratsinsham—operatedgroupwereinjectedwithnormalsaline(NS),andtheratsinothergroupswereinjec
7、tedwithAp25—35inhippocampus.Theratsweregivencorrespondingdrugbygavageonceadayfor30daysaftersurgery.Therats’learningandmemorizingabilitywereevaluatedbytheMorriswatermaze.ThentheyweresacrificedandtheirbrainsweretakenfordeterminationofthecorticalGSK一3Band
8、CDK一5expressionbyimmunohistochemistryandwesternblotting.Results:HuanglianJieduDecoctiondecreasedthemeanescapelatencyindirectionalnavigationtest(P<0.05,P<0.01),increasedthetimesoftraversingplatform(P<0.05),andincreasedtheeffectivequadran
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