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1、·38InfeetInflammRepVol1No1June2000一‘严重烫伤后高迁移率族蛋白1基因表达的改变及意义2方文慧姚咏明施志国于燕100037北京解放军第304医院烧伤研究所基础部“吴叶陆连荣盛志勇:、一【摘要】目的观察烫伤后肝肺组织高迁移率族蛋白l(HMG-1)mRNA表达的变化规律及其与器官功能。:,、损害的关系方法采用大鼠35%nlo烫伤模型动物随机分为正常对照组(n一7)烫伤组(n一24)和重组杀菌/、21,通透性增加蛋白(rBPI)治疗组(n~12)留取组织和血标本分别检测组织内毒素含量HMGI功RNA表达及器。.:、,官功能指标结果严重烫伤后早期肝肺
2、组织HMG-1基因表达改变不明显伤后24小时则明显增多(尸<005..。,Z:,一001)且一直持续至伤后72小时(尸<001)给予rBPI治疗可有效防治肠源性内毒素移位的发生并显著、。,、抑制肝肺组织HMGImRNA水平相关分析结果显示肝组织HMGImRNA表达与血清谷丙转氨酶谷草..。、:转氨酶水平肺组织HMGI扭RNA表达与髓过氧化物酶活性呈显著正相关(P<005一。01)结论严重烫伤、,,后肝肺组织HMG-1表达显著增多且持续时间较长局部组织HMGI诱生与烧伤后内毒素介导器官功能损害。关系密切一关键词烧伤高迁移率族蛋白1基因表达内毒素类重组蛋白质类Thesiifle
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5、samplesfromliverandlungswereeolleetedtomeasuretissueendotoxinlevelsandHMIG..exressonnaon,oosaesereoaneoreasureenooraneoaraeers-mRNApiIdditibldmplwbtidfmmtfgfuntinpmtResu:sneanereaseoeeeressonssuesasoserveaoursosurn,anre-ltsAigifitinfHMIgnxpiintiwbdt24hPtbditG...aneareeevaeuoorsaereaur一reae
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7、005001)ConelusionsThmlinjyPnmkdlynhnG.eeeressovarousorans一reuaonoeressoaevoveeaoene-HMIgnxpininigUPgltifHMIxPinmybinldinthPthgGG,55oenooxn一eaeueoranaaesearoorurns1fdtimditdmltiplgdmgcondytmajbeorursorou一roeeneexressoeeoaroesooxnKywdsBnHighmbilitygp1ptinGpinRmb
