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时间:2020-04-23
《β-羟基异戊酰紫草素联合顺铂对SKOV3细胞活力的影响-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中国中西医结合杂志2014年8月第34卷第8期CJITWM,August2014,Vo1.34,No.8I3一羟基异戊酰紫草素联合顺铂对SKOV3细胞活力的影响张洁沈敏鹤阮善明摘要目的观察B.羟基异戊酰紫草素(13一HIVS)联合顺铂对人卵巢癌细胞株SKOV3活力的影响,并探讨其可能的发生机制。方法设立空白对照组及6个不同浓度13一HIVS组(2~30~mol/L),应用四甲基偶氮唑蓝(M-I-r)比色法测定不同浓度B.HIVS对SKOV。细胞活力的影响。设立顺铂单用组(1O、20、4OFmollL)和选择2、25、1、2.5~,mol/L)协同顺铂组,应用M1_r比色法测定13.HlVS协同顺铂对SKOV细胞活力的影响。应用Westernblot法检测不同浓度13一HIVS作用后BcI一2、Bax蛋白表达。结果不同浓度13一HIVS(2~30~mol/L)均可抑制SKOV。细胞活力,并呈一定的浓度依赖性,测得半数抑制率lC。为7.37m0l/L,与空白对照组比较,差异均有统计学意义(P<0.05)。3-HIVS(1、2.5m0I/L)与顺铂联合应用,与顺铂单用组比较,差异亦有统计学意义(P<0.05,P<0.01)。协同作用对B—HIVS呈现浓度依3、赖性。Westernblot检测结果表明:不同浓度13一HIVS(5、7.5、10mol/L)可明显上调SKOV细胞中Bax蛋白表达水平,同时抑制Bc1.2蛋白表达,与对照组比较,差异有统计学意义(P4、;生长抑制;细胞凋亡;联合疗法13.HIVSCombinedCisplatinInhibitedActivitiesofHumanOvarianCancerCelILineSKOV3/nvitroZHANGJje.SHENMin—he,andRUANShan-mingDepartmentofTumor,FirsfAffiliatedHospital,ZhejiangHospitalofTraditionafChineseMedicine,Hangzhouf310006),ChinaABSl1。fACTObiectiveTostudytheeff5、ectofB.hydroxyisovalerylshikonin(B—HIVS)combinedcisplatinonactivitiesofovariancancercelIIineSKOVinvivoanditspossiblemechanisms.MethodsCellsweredividedintotheblankcontrolgroupandsixB-HIVSgroups(2-30/~mollL).Effectof13-HIVSatdifferentcOncentratiOnsontheactivitiesofovariancance6、rcelIlineSKOVwasdetectedusingM3-1-assay.SKOVscellsweretreatedwithcisplatin(10,20,and40p,mol/L)and13一HIVS(0.25,1,and2.5t~mol/L)combinedcisplatin.Effectof6.HIVScombinedcisplatinontheactivitiesofovariancancercelIlineSKOV3wasdeterminedbyM-i-rassay.TheexpressionofBcl-2andBaxafter7、treatedbydifferentconcentrationsof13一HIVSwasdetectedbyWesternblot.ResultsTheactivitiesofSKOV3wereinhibitedbydifferentcon—centrationsof13.HlVSdose—dependently.The50%inhibitionrate(IC50)was7.37i~mol/L.Therewassta—tisticaldifferenceinIC5Qbetweeneachconcentration13一HIVSgroupandt8、heblankcontrolgroup(尸<0.05).Therewasstatisticaldi仟erenceinIC50betweenthe13一
2、25、1、2.5~,mol/L)协同顺铂组,应用M1_r比色法测定13.HlVS协同顺铂对SKOV细胞活力的影响。应用Westernblot法检测不同浓度13一HIVS作用后BcI一2、Bax蛋白表达。结果不同浓度13一HIVS(2~30~mol/L)均可抑制SKOV。细胞活力,并呈一定的浓度依赖性,测得半数抑制率lC。为7.37m0l/L,与空白对照组比较,差异均有统计学意义(P<0.05)。3-HIVS(1、2.5m0I/L)与顺铂联合应用,与顺铂单用组比较,差异亦有统计学意义(P<0.05,P<0.01)。协同作用对B—HIVS呈现浓度依
3、赖性。Westernblot检测结果表明:不同浓度13一HIVS(5、7.5、10mol/L)可明显上调SKOV细胞中Bax蛋白表达水平,同时抑制Bc1.2蛋白表达,与对照组比较,差异有统计学意义(P4、;生长抑制;细胞凋亡;联合疗法13.HIVSCombinedCisplatinInhibitedActivitiesofHumanOvarianCancerCelILineSKOV3/nvitroZHANGJje.SHENMin—he,andRUANShan-mingDepartmentofTumor,FirsfAffiliatedHospital,ZhejiangHospitalofTraditionafChineseMedicine,Hangzhouf310006),ChinaABSl1。fACTObiectiveTostudytheeff5、ectofB.hydroxyisovalerylshikonin(B—HIVS)combinedcisplatinonactivitiesofovariancancercelIIineSKOVinvivoanditspossiblemechanisms.MethodsCellsweredividedintotheblankcontrolgroupandsixB-HIVSgroups(2-30/~mollL).Effectof13-HIVSatdifferentcOncentratiOnsontheactivitiesofovariancance6、rcelIlineSKOVwasdetectedusingM3-1-assay.SKOVscellsweretreatedwithcisplatin(10,20,and40p,mol/L)and13一HIVS(0.25,1,and2.5t~mol/L)combinedcisplatin.Effectof6.HIVScombinedcisplatinontheactivitiesofovariancancercelIlineSKOV3wasdeterminedbyM-i-rassay.TheexpressionofBcl-2andBaxafter7、treatedbydifferentconcentrationsof13一HIVSwasdetectedbyWesternblot.ResultsTheactivitiesofSKOV3wereinhibitedbydifferentcon—centrationsof13.HlVSdose—dependently.The50%inhibitionrate(IC50)was7.37i~mol/L.Therewassta—tisticaldifferenceinIC5Qbetweeneachconcentration13一HIVSgroupandt8、heblankcontrolgroup(尸<0.05).Therewasstatisticaldi仟erenceinIC50betweenthe13一
4、;生长抑制;细胞凋亡;联合疗法13.HIVSCombinedCisplatinInhibitedActivitiesofHumanOvarianCancerCelILineSKOV3/nvitroZHANGJje.SHENMin—he,andRUANShan-mingDepartmentofTumor,FirsfAffiliatedHospital,ZhejiangHospitalofTraditionafChineseMedicine,Hangzhouf310006),ChinaABSl1。fACTObiectiveTostudytheeff
5、ectofB.hydroxyisovalerylshikonin(B—HIVS)combinedcisplatinonactivitiesofovariancancercelIIineSKOVinvivoanditspossiblemechanisms.MethodsCellsweredividedintotheblankcontrolgroupandsixB-HIVSgroups(2-30/~mollL).Effectof13-HIVSatdifferentcOncentratiOnsontheactivitiesofovariancance
6、rcelIlineSKOVwasdetectedusingM3-1-assay.SKOVscellsweretreatedwithcisplatin(10,20,and40p,mol/L)and13一HIVS(0.25,1,and2.5t~mol/L)combinedcisplatin.Effectof6.HIVScombinedcisplatinontheactivitiesofovariancancercelIlineSKOV3wasdeterminedbyM-i-rassay.TheexpressionofBcl-2andBaxafter
7、treatedbydifferentconcentrationsof13一HIVSwasdetectedbyWesternblot.ResultsTheactivitiesofSKOV3wereinhibitedbydifferentcon—centrationsof13.HlVSdose—dependently.The50%inhibitionrate(IC50)was7.37i~mol/L.Therewassta—tisticaldifferenceinIC5Qbetweeneachconcentration13一HIVSgroupandt
8、heblankcontrolgroup(尸<0.05).Therewasstatisticaldi仟erenceinIC50betweenthe13一
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