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时间:2020-04-02
《二烯丙基二硫对人白血病HL_60细胞凋亡启动过程的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、《癌症》ChineseJournalofCancer,2009,28(1):43-4843·基础研究·二烯丙基二硫对人白血病HL-60细胞凋亡启动过程的影响易岚,曾希,谭晖,葛玲,吉小霞,林敏,苏琦ProteomicsanalysisofapoptosisinitiationinducedbydiallyldisulfideinhumanleukemiaHL-60cellsLanYi,XiZeng,HuiTan,LingGe,Xiao-XiaJi,MinLin,QiSu[Abstract]BackgroundandObjective:Diallyldisulfi
2、de(DADS),anantitumorreagent,hasincreasinglygainedattention.ThisstudywastoexploretherelatedproteinsinDADS-inducedapoptosisinitiationinhumanleukemiaHL-60cells.Methods:TotalproteinofHL-60cellswithorwithout2-daytreatmentof3.6mg/LDADSwasextracted,separatedbytwo-dimensionalpolyacrylamidege
3、lelectrophoresis,andanalyzedbyPDQuest2-DEsoftware.Differentiallyexpressedproteinswereseparatedandidentifiedbypeptidemassfingerprintinganalysisandbioinformatics,andverifiedbyWesternblotandreversetranscription-polymerasechainreaction(RT-PCR).Results:AscomparedwiththoseinuntreatedHL-60c
4、ells,29proteinswere南华大学differentiallyexpressedinDADS-treatedHL-60cells:22wereup-肿瘤研究所,regulatedandsevenweredown-regulated.Amongnineproteinswhichwere湖南衡阳421001randomlyselectedforpeptidemassfingerprintinganalysisandbioinformatics,sevenweremeaningful.Theseproteinswereassociatedwithcellu
5、larresponses,CancerResearchInstitute,genetranscriptionandregulation,cytoskeleton,metabolism,andsoon.UniversityofSouthChina,WesternblotshowedthatRas-relatedC3botulinumtoxinsubstrate2(Rac2)Hengyang,Hunan,421001,wasup-regulatedinDADS-treatedHL-60cells;thisresultwasaccordantwithP.R.China
6、RT-PCRresults.Conclusion:Someproteins,includingRac2,maybeinvolvedinDADS-inducedapoptosisinhumanleukemiaHL-60cells,butthe通讯作者:苏琦exactmechanismneedstobeexplored.Correspondenceto:QiSuKeywords:diallyldisulfide,leukemia,HL-60cell,apoptosis,proteomics,Tel.:86.734.8281547Rac2Fax:86.734.8281
7、547Email:suqi@hotmail.com【摘要】背景与目的:二烯丙基二硫(diallyldisulfide,DADS)作为一种抗肿瘤物质日益受到关注。本研究在建立DADS启动HL-60细胞凋亡模型的基础上,研基金项目:湖南省自然科学基金究DADS启动人白血病HL-60细胞凋亡的相关蛋白。方法:提取未处理HL-60细(No.07JJ6155);湖南省教育厅胞和3.6mg/LDADS处理2d的HL-60细胞的总蛋白,双向电泳分离细胞总蛋白科研项目(No.06C696)质,绘制图谱,PDQuest软件分析,切割差异蛋白质,进行质谱分析、生物信息学分析,Wes
8、ternblot以及RT
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