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ID:51948873
大小:83.00 KB
页数:12页
时间:2020-03-20
《骨髓间充质干细胞促进骨-肌腱结合部愈合初步观察.doc》由会员上传分享,免费在线阅读,更多相关内容在工程资料-天天文库。
1、骨髓间充质干细胞促进骨-肌腱结合部愈合初步观察【摘要】观察骨髓间充质干细胞对骨-肌腱结合部愈合的影响。[方法]采用骨髓穿刺、全骨髓培养法获取兔骨髓间充质干细胞。36只18周龄新西兰大白兔随机分为2组,实验组将骨髓间充质干细胞与PluronicF-127载体材料结合后,植入兔離骨部分切除模型,对照组只进行手术,不植入细胞。在术后6、12、18周进行X线片、大体和组织学观察评估。[结果]X线片显示术后6、12、18周实验组骨-肌腱结合部新生骨面积显著大于对照组(P<;0.01)o大体观察可见实验组骨-腱结合部愈合较早。HE染色显
2、示实验组术后6周有大量的纤维母细胞和类软骨细胞增生,并可见新骨形成;12周,韻腱与松质骨接触面软骨细胞移行带形成;18周,纤维软骨移行带排列更有序。Safranin0染色显示实验组术后6周,基质染色较深,部分骨-腱结合处可见相对浓染区;12周,基质染色范围明显减少,沿类软骨细胞走行分布;18周,类软骨细胞集中的区域染色较12周有所减退。组织学检查显示实验组术后6、12、18周骨-腱结合部组织愈合明显,较对照组迅速。[结论]骨髓间充质干细胞可以促进骨-肌腱结合部细胞增生,促进其早期愈合。【关键词】骨髓间充质干细胞骨-肌腱结合部愈合
3、Abstract:[Objective]ToevaluatethoeffectsofBoneMesenchymalStemCells(BMSCs)onhealingofbonetendonjunctioninapatellartendoncomplex・[Method]TheBMSCsofrabbitswereharvestedbybonemarrowaspiration.AdherentcellswereselectedasBMSCsafterthewholemarrowwascultured・Standardpartialp
4、atellectomywasconductedin3618weekoldrabbitsdividedinto2groups・TheexperimentalgroupwasplantedintobythecompositeofBMSCsandPluronicF127whilethecontrolgroupwasonlyoperatedwithoutBMSCsplantinginto.Thepatellarpatellartendoncomplexeswereharvestedatweeks6,12,18postoperativel
5、yforradiographic,grossandhistologicalevaluations・[ResuIt]Radiographicmeasurementsshowedsignificantlymorenewlyformedboneatthepatellartendonpatellarhealingjunctionintheexperimentedgroupcomparedwiththatofthecontrolsatweeks6,12,18(P<;0.01).Thegrossobservationsshowedthe
6、tissuehealingintheexperimentalgroupwasquickerthanthatofthecontrols・Histologically,HEstainedspecimenshowedintheexperimentalgroupconsiderablefibroblastandchondrocytelikecellularproliferation,newbonesformedatweek6;chondroeytestransitionalzonewasformedattrabecularbonepat
7、ellartendonjunctionatweek12;arrayoffibrocartilagetransitionalzonewasorderedatweek18.Safranin0stainedspecimenshowedintheexperimentaigroupmatrixwasstainedredatnewlyformedbonetopatellartendonjunction,partiallythickatweek6;matrixstainsreducedanddistributedalongchondrocyt
8、elikecellsatweek12,stainsintherealmofchondrocytelikecellsconcentrationwasreducedthanbeforeatweek18.Histologicalobservationsshowedmo
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