资源描述：
《冠状病毒Coronavirusl论文-2008 Detection of Antigenic Heterogeneity in Feline Coronavirus Nucleocapsid in Feline Pyogranulomatous Meningoencephali.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、InfectiousDiseaseVetPathol45:140–153(2008)DetectionofAntigenicHeterogeneityinFelineCoronavirusNucleocapsidinFelinePyogranulomatousMeningoencephalitisL.PONCELET,A.COPPENS,D.PEETERS,E.BIANCHI,C.K.GRANT,ANDH.KADHIMAnatomy/EmbryologyDepartment,FacultyofMedicine,FreeUniversityofBrussels,Bruxelles,Be
2、lgium(LP,AC);ClinicalSciencesDepartment,FacultyofVeterinaryMedicine,UniversityofLie`ge,Lie`ge,Belgium(DP);AnimalHealthDepartment,FacultyofVeterinaryMedicine,UniversityofParma,Parma,Italy(EB);CustomMonoclonalsInternational,Sacramento,CA(CG);andAnatomicPathologyDepartment;NeuropathologyUnit,Brugm
3、annUniversityHospitalandChildrenHospital,FreeUniversityofBrussels,Bruxelles,Belgium(HK)Abstract.Anewmonoclonalantibody(mAb),CCV2-2,wascomparedwiththewidelyusedFIPV3-70mAb,bothdirectedagainstcaninecoronavirus(CCoV),asadiagnosticandresearchtool.Westernblotshowedthatbothanti-CCoVmAbsonlyreactedwit
4、haproteinof50kD,aweightconsistentwiththefelinecoronavirus(FCoV)viralnucleocapsid.Acompetitiveinhibitionenzyme-linkedimmunosor-bentassayshowedthatthe2recognizedepitopesaredistinct.PreincubationofCCV2-2mAbwithFCoVantigensuppressedtheimmunostaining.Formalin-fixed,paraffin-embeddedsectionsfrombrain
5、sof15catswiththedryformoffelineinfectiousperitonitis(FIP)wereexaminedbyimmunohistochemistry.Immunohistochemistrywasperformedwithbothanti-CCoVmAbs,eitheronconsecutiveoronthesamesections.Amyeloid-histiocyticmarker,MAC387,wasalsousedtoidentifyFIPvirus–infectedcells.InallregionswhereMAC387–positive
6、cellswerepresent,positivestainingwiththeCCV2-2mAbwassystematicallydetected,exceptatsomelevelsin1cat.Incontrast,noneoronlyafewcellswerepositivefortheFIPV3-70mAb.DoubleimmunostainingshowedmacrophagesthatwereimmunopositiveforeitherCCV2-2aloneoralternativelyforCCV2-2andFIPV3-70mAbs.Thisrevealstheco
7、existenceof2cohortsofphagocyteswhoseFIPviralcontentsdifferedbythepresenceorabsenceoftheFIPV3-70–recognizedepitope.ThesefindingsprovideevidenceforantigenicheterogeneityincoronavirusnucleocapsidproteininFIPlesions,aresultthatisinlin
