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1、JournalofGeneralVirology(2006),87,1691–1695DOI10.1099/vir.0.81749-0AnalysisofACE2inpolarizedepithelialcells:ShortCommunicationsurfaceexpressionandfunctionasreceptorforsevereacuterespiratorysyndrome-associatedcoronavirus1123XiaofengRen,Jo¨rgGlende,MarwanAl-Falah,VictordeVries,1443ChristelSchwegmann-W
2、essels,XiuxiaQu,LeiTan,ThomasTschernig,421HongkuiDeng,HassanY.NaimandGeorgHerrler1,2Institutfu¨rVirologie1andInstitutfu¨rPhysiologischeChemie2,Tiera¨rztlicheHochschuleCorrespondenceGeorgHerrlerHannover,Bu¨nteweg17,D-30559Hannover,GermanyGeorg.Herrler@tiho-hannover.de3Institutfu¨rfunktionelleundangew
3、andteAnatomie,MedizinischeHochschuleHannover,Carl-Neuberg-Straße,D-30625Hannover,Germany4DepartmentofCellBiologyandGenetics,CollegeofLifeSciences,PekingUniversity,Beijing100871,People’sRepublicofChinaTheprimarytargetofsevereacuterespiratorysyndrome-associatedcoronavirus(SARS-CoV)isepithelialcellsint
4、herespiratoryandintestinaltract.ThecellularreceptorforSARS-CoV,angiotensin-convertingenzyme2(ACE2),hasbeenshowntobelocalizedontheapicalplasmamembraneofpolarizedrespiratoryepithelialcellsandtomediateinfectionfromtheapicalsideofthesecells.Here,theseresultswereconfirmedandextendedbyincludingacoloncarcin
5、omacellline(Caco-2),alungcarcinomacellline(Calu-3)andVeroE6cellsinouranalysis.AllthreecelltypesexpressedhumanACE2ontheapicalmembranedomainandwereinfectedviathisroute,asdeterminedwithvesicularstomatitisviruspseudotypescontainingtheSproteinofSARS-CoV.Inahistologicalanalysisoftherespiratorytract,ACE2wa
6、sdetectedinthetrachea,Received8December2005mainbronchusandalveoli,andoccasionallyalsointhesmallbronchi.ThesedatawillhelpusAccepted1February2006tounderstandthepathogenesisofSARS-CoVinfection.Epitheliaareaprimarybarriertoinfectionbymicro-respiratorybutalsotheintestinalepitheliumissusceptibleorganismse
7、nteringtheirhostviabodycavitiessuchasthetoinfection.Ithasbeenshownrecentlythatthereceptorforrespiratoryorintestinaltract(reviewedbyCompans&SARS-CoV,angiotensin-convertingenzyme2(ACE2;LiHerrler,2005).E
