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1、JOURNALOFVIROLOGY,Sept.2006,p.89208928Vol.80,No.180022-538X/06/$08.000doi:10.1128/JVI.00649-06Copyright©2006,AmericanSocietyforMicrobiology.AllRightsReserved.ExtremelyLowExposureofaCommunitytoSevereAcuteRespiratorySyndromeCoronavirus:FalseSeropositivit
2、yduetoUseofBacteriallyDerivedAntigens11,23441D.T.M.Leung,W.W.C.vanMaren,F.K.L.Chan,W.S.Chan,A.W.I.Lo,C.H.Ma,145,63,61F.C.H.Tam,K.F.To,P.K.S.Chan,J.J.Y.Sung,andP.L.Lim*12ClinicalImmunologyUnit,TheChineseUniversityofHongKong,HongKong;UniversityofUtrecht,U
3、trecht,TheNetherlands;3DepartmentofMedicineandTherapeutics,TheChineseUniversityofHongKong,HongKong;DepartmentofAnatomicaland4CellularPathology,TheChineseUniversityofHongKong,HongKong;DepartmentofMicrobiology,TheChineseUniversityof56HongKong,HongKong;and
4、CentreforEmergingInfectiousDiseases,TheChineseUniversityofHongKong,HongKongReceived31March2006/Accepted20June2006Estimatesofseropositivitytoanewinfectiousagentinacommunityareusefultopublichealth.Forsevereacuterespiratorysyndrome(SARS),thefiguresareconflic
5、ting.Herein,wescreened12,000peopleinacommunitystrickenbySARS10monthspreviouslyandfound53individuals(0.44%)whohadimmunoglobulinGantibodiestotheSARScoronavirus(SARS-CoV)nucleocapsid(N)producedinbacteria.However,onlysevenofthese(group1)hadserawhichalsoreac
6、tedwiththenativeNantigenexpressedinSARS-CoV-infectedVerocells,N-transfected293Tcells,andtissuesofinfectedSARSpatients.Ofthese,sixindividualshadhadSARSpreviously.Theremainingperson,aswellasthe46otherindividuals(group2),werehealthyandhadnohistoryofSARS.Gr
7、oup1antibodiesrecognizedepitopeslocatedslightlydifferentlyinNfromthoseofgroup2antibodies,andamousehybridomaantibodyresemblingtheformertypewasgenerated.Unusually,group2antibodiesappearedtorecognizecross-reactivebacterialepitopesthatpresumablywereposttran
8、slationallymodifiedineukaryotesandhencewereprobablynotinducedbySARS-CoVorrelatedcoronavirusesbutratherbybacteria.TheNantigenisthushighlyunique.Theextremelylowrate(0.008%)ofasymptomaticSARSinfectionfoundatteststothehighvirulenceoft
