冠状病毒Coronavirusl论文-1990 Four major antigenic sites of the coronavirus transmissible gastroenteritis virus are located on the amino-terminal.pdf

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1、JournalofGeneralVirology(1990),71,1313-1323.PrintedinGreatBritain1313Fourmajorantigenicsitesofthecoronavirustransmissiblegastroenteritisvirusarelocatedontheamino-terminalhalfofspikeglycoproteinSBernardDeimas,DenisRasschaert,MurielleGodet,JacquelineGelfiandHubertLaude*LaboratoiredeViro

2、logieetd'ImmunologieMol~culaires,InstitutNationaldelaRechercheAgronomique,CentredeRecherchesdeJouy-en-Josas,78350Jouy-en-Josas,FranceFourmajorantigenicsiteshavebeendelineatedontheendopeptidases.Twoidentifiedcleavageproductsofspikeprotein(S)oftheporcineentericcoronavirus26Kand13K,immun

3、oreactivetositeA-B-andsitetransmissiblegastroenteritisvirus(TGEV)inpreviousD-specificMAbsrespectively,couldbealignedontheStopologicalstudiesusingmonoclonalantibodiesprimarystructureaccordingtoN-terminalsequence(MAbs).Correlationofthesesiteswiththephysicaldata.Thisledustoproposethatthe

4、majorneutral-structureoftheproteinwasachievedbyuseofdifferentizationdomainA-Biscontainedinaregionofapproaches.RecombinantpEXplasmidsdirectingtheapproximately200residueswithresidue506asitsNsynthesisofvariousfusedSpolypeptideswerecon-boundary.Similarly,siteDepitopesshouldbelocatedstruct

5、ed.AhybridproteincontainingnineS-specificwithinastretchof130residues,startingat82residuesresidues(363to371)wasshowntoexpresssiteCfromtheNterminus.Pointmutationsidentifiedbyepitopes.TheothersiteswerelocalizedthroughstudydirectRNAsequencingofneutralization-resistantoftheantigenicactivit

6、yoffragmentsgeneratedbymutantswereconsistentwiththeproposedlocationofcontrolledcleavageofthenativeproteinwithdifferentthesesites.IntroductionMajorantigenicsites,designatedA,B,CandD,havebeendelineatedusingthelibraryofMAbsestablishedinTransmissiblegastroenteritisvirus(TGEV)causesanourla

7、boratory.Mostoftheepitopescriticalforneutral-acuteandusuallyfatalentericdiseaseinnewbornpiglets.izationwerefoundtobeclusteredintheimmunodomin-Togetherwiththemurinehepatitisvirus(MHV)andantAandBsites,buttheothertwositescontainedinfectiousbronchitisvirus(IBV),TGEVisoneofthesubsidiaryneu

8、trali