冠状病毒Coronavirusl论文-1980 A two year serological surveillance of coronavirus infections in Hamburg.pdf

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1、D.E.Sarateanu,W.EhrengutATwoYearSerologicalSurveillanceofCoronavirusInfectionsinHamburgAssomecasesdidnotappeartobelinkedwithinfectionsbyknownviruses,itwashopedthatthehumancoronavi-rusesdiscoveredrelativelyrecentlymightaccountforaproportionoftheseunexplai

2、nedillnesses.Thatcoronavirusesarepotentialrespiratorypathogenshasbeendemonstratedinexperimentalstudies(1,2).However,dataontheextentandsignificanceofinfectionsbythesevirusesinhumanillnessesarestilllimited.Serologicalsurveillancecarriedoutatregularinterval

3、sonalargenumberofseratakenfromrepresentativegroupsrevealsthetotalinfectionrates(apparentandnon-appar-ent)andthelevelanddistributionoftheseroimmunityinthepopulation.Thefollowingreportofseroepidemiologicalstudiesofcoronavirusinfectionsisbaseduponobservatio

4、nsmadeinthecourseofasurveillanceprogrammeinHamburg(carriedoutsinceOctober1974)invarioussectionsofthepopulationinwhichallagegroupswererepresented.MaterialandMethodsPopulation:Atotalof3,016bloodsampleswascollectedbe-tweenOctober1974andSeptember1976(minimum

5、of100seramonthly)from"normal"healthyindividualsdividedintofourage-groups:GroupI(0to14yearsold);GroupII(15to24yearsold);GroupIII(25to59yearsold);GroupIV(60yearsold).Wecollectedasecondsampleofbloodtwomonthslaterfrom331girls,12to17yearsold.Afterinactivation

6、for30minutesat56°C,theserawerestoredat-20°Cuntilused.Allpairedseraweretestedsimultaneously.Antigen:CoronavirusstrainOC-43hasbeenisolatedbyMcln-toshetal.(3)inhumanembryotracheaorganculturefromanadultwithacold.Thestrainwassubsequentlyadaptedtosucklingmouse

7、brain.StrainOC-43antigenwaspreparedfrominfectedsucklingmousebrainaccordingtothetechniquedescribedbyKayeandDowdle(4).Serology:Hemagglutinationinhibition(HI)testswereper-formedaccordingtotheSevermicrotitretechnique(5),usingchickenredbloodcells(4).Fiftyposi

8、tivesera(titre1:16-1:64)weretreatedwithreceptordestroyingenzyme(RDE)(6),kaolin(7)orethacridin(8)forremovalofnonspecificinhibitors.ResultsTheincidenceofseropositivereactionsinthetotalpopu-lationstudiedispresentedinTable1.Fr