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1、EnzymaticSynthesisandAntioxidantPropertiesofPoly(rutin)(芦丁)的酶促合成与抗氧化性质MotoichiKurisawa:siAbstract摘要Rutin,quercetin-3-rutinoside,isoneofthemostfamousglycosidesofflavonoidandwidelypresentinmanyplants.Inthisstudy,weperformedanoxidativepolyme
2、rizationofnitinusingMyceliophthoralaccaseascatalystinamixtureofmethanolandbuffertoproduceaflavonoidpolymerandevaluatedantioxidantpropertiesoftheresultantpolymer.Underselectedconditions,thepolymerwithmolecularweightofseveralthousandswasobtainedingoodyields.Theresultingpolymerwasreadil
3、ysolubleinwater,DMF,andDMSO,althoughrutinmonomershowedverylowwatersolubility.UVmeasurementshowedthatthepolymerhadbroadtransitionpeaksaround255and350nminwater,whichwerered-shiftedinanalkalinesolution.Electronspinresonance(ESR)measurementshowedthepresenceofaradicalinthepolymer.Thepolym
4、ershowedgreatlyimprovedsuperoxidescavengingactivityandinhibitioneffectsonhumanlow-densitylipoprotein(LDL)oxidationinitiatedby2,2'-azobis(2-amidino-propane)dihydrochloride(AAPH),comparedwiththerutinmonomer.ThepolymeralsoprotectedendothelialcellsfromoxidativeinjuryinducedbyAAPHasaradic
5、algeneratorwithamuchgreatereffectthanthenitinmonomer.芦丁,榊皮素・3■芸香糖苜,是最著名的黄酮类糖昔之一,广泛存在于很多植物中。在本研究中,我们用毁丝酶属漆酶做催化剂,在甲醇和缓冲剂的混合物中进行了芦丁的氧化聚合,得到了一个黄酮类聚合物并评价了生成聚合物的抗氧化性质。在选定的条件下,分子量为几千的聚合物的产率较好。尽管芦丁单体显示出很低的水溶性,但其生成的聚合物可以很好地溶于水,DMF及DMSO。UV测量显示,该聚合物溶于水在255nm和350nm处有较宽的跃迁峰,这是在碱性溶液中红移的结果。电子自旋共振测量
6、显示,该聚合物中有一个自由基。与芦丁单体相比,该聚合物通过2,21偶氮(2■甲基丙基眯)•二盐酸盐(AAPH)的诱导,显示出较强的超氧化物清除活性,和较强的对人体低密度脂蛋白的氧化的抑制作用。AAPH作为自由基发生器,可诱导该聚合物保护内皮细胞免受氧化伤害,比芦丁单体的作用更强。Introduction简介Recentinterestinflavonoidshasincreasedgreatlyduetotheirbiologicalandphannacologicalactivityincludingantioxidant,anticarcinogenic,p
7、robiotic,antimicrobial,andantiinflammatoryproperties.1Theflavonoidsconsistofalargegroupoflowmolecularweightpolyphenolicsubstances,naturallyoccurringinfruits,vegetables,tea,andwine,andareanintegralpartofthehumandiet.最近,由于黄酮类的生物和药理活性,包括抗氧化,抗癌,益生素,抗菌和抗感染等活性,人们对类黄酮的研究兴趣大大增加。黄酮类化合物由大量低分子量
8、的多酚类物质组成,天然存