资源描述：
《葡和樱基因表达差异》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、园艺学报2009,36(1):87-92ActaHorticulturaeSinica茶树β-葡萄糖苷酶和β-樱草糖苷酶基因表达差异分析333陈亮,赵丽萍,马春雷,王新超,姚明哲(中国农业科学院茶叶研究所,茶树资源与改良研究中心/国家茶树改良中心,杭州310008)摘要:β-葡萄糖苷酶和β-樱草糖苷酶是茶叶香气释放过程中2个重要的内源性水解酶。采用实时定量PCR技术,分析了‘龙井43’茶品种春季不同嫩度新梢,以及8个不同品种春季一芽二叶的β-葡萄糖苷酶和β-樱草糖苷酶基因的表达差异。结果表明,β-葡萄糖苷酶基因表达量以一芽三叶最高,为7-15183×10cop
2、y·μL,一芽三叶>一芽一叶>一芽二叶>一芽四叶;β-樱草糖苷酶基因在一芽一叶表达6-1量很低,一芽二叶最高(2149×10copy·μL),而后随着成熟度的增加而降低。β-葡萄糖苷酶基因的表达量明显高于β-樱草糖苷酶基因,一芽二叶和一芽三叶新梢中两个基因的表达量均较高。8个不同品种中,‘福鼎大白茶’2个基因表达量均非常高。8个品种β-葡萄糖苷酶基因表达量是β-樱草糖苷酶的2016(214～4516)倍。不过,‘龙井43’不同时空、不同茶树品种β-葡萄糖苷酶活性测定结果与基因表达量之间的相关性均不明显。关键词:茶树;香气;基因;表达差异中图分类号:S57111
3、文献标识码:A文章编号:05132353X(2009)0120087206DifferentialExpressionAnalysisofβ2glucosidaseandβ2primeverosidaseGenesinTeaPlant(Camelliasinensis)333CHENLiang,ZHAOLi2ping,MAChun2lei,WANGXin2chao,andYAOMing2zhe(ResearchCenterforTeaGermplasmandImprovement,TeaResearchInstituteChineseAcademyofAgri
4、culturalSciences;NationalCenterforTeaImprovement,Hangzhou310008,China)Abstract:β2glucosidaseandβ2primeverosidasearetwoimportantendogenoushydrolyticenzymesfortheformationofteaaroma.Real2timePCRtechniquewasemployedtoanalyzethedifferentialexpressionoftwoa2romarelatedgenes,β2glucosidase
5、andβ2primeverosidasegenes,indifferentmaturityshootsanddifferentsourcecultivarsinspringseasonofteaplant(Camelliasinensis).Theresultsindicatedthatthehighestex27-1pressionlevelforβ2glucosidasegenewasfoundin‘threeandabud’(5183×10copy·μL).Theexpres2sionlevelorderofβ2glucosidasegenewas‘th
6、reeandabud’>‘oneandabud’>‘twoandabud’>‘fourandabud’.Forβ2primeverosidasegene,expressionlevelin‘oneandabud’wasverylow,thehigh26-1estwasfoundin‘twoandabud’(2149×10copy·μL).Theexpressionleveldecreasedwithyoungshootbecomingmature.Theexpressionlevelofβ2glucosidasegeneishighercomparedwith
7、thatofβ2primeverosi2dasegeneinteaplant.Bothgenesarehighlyexpressedin‘twoandabud’or‘threeandabud’shoots.Thegenesexpressionlevelof8teacultivars,whicharetypicalclonessuitableforgreen,OolongandblackteasfromChina,JapanandKenya,wereassessed.BothgeneswereveryhighlyexpressedinFudingDabaicha
8、.Theratioofexpressi
