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1、SerumMetabonomicAnalysisofProtectiveEffectsofCurcumaaromaticaOilonRenalFibrosisRats1.1.3.111LiangcaiZhao,HaiyanZhang,YunjunYang,YongquanZheng,MinjianDong,YaqiangWang,2221,2GuanghuiBai,XinjianYe,ZhihanYan*,HongchangGao*1InstituteofMetabonomics&MedicalNMR,SchoolofPharmaceuticalSciences,WenzhouMed
2、icalUniversity,Wenzhou,China,2RadiologyDepartmentoftheSecondAffiliatedHospital,WenzhouMedicalUniversity,Wenzhou,China,3DepartmentofRadiology,thefirstAffiliatedHospital,WenzhouMedicalUniversity,Wenzhou,ChinaAbstractBackground:Curcumaaromaticaoilisatraditionalherbalmedicinedemonstratingprotective
3、andanti-fibrosisactivitiesinrenalfibrosispatients.However,studyofitsmechanismofactionischallengedbyitsmultiplecomponentsandmultipletargetsthatitsactiveagentactson.Methodology/PrincipalFindings:Nuclearmagneticresonance(NMR)-basedmetabonomicscombinedwithclinicalchemistryandhistopathologyexaminati
4、onwereperformedtoevaluateinterveningeffectsofCurcumaaromaticaoilonrenalinterstitialfibrosisratsinducedbyunilateralureteralobstruction.Themetabolitelevelswerecomparedbasedon1integralvaluesofserumHNMRspectrafromratson3,7,14,and28daysafterthemedicineadministration.Timetrajectoryanalysisdemonstrate
5、dthatmetabolicprofilesoftheagent-treatedratswererestoredtocontrollevelsafter7daysofdosage.Theresultsconfirmedthattheagentwouldbeaneffectiveanti-fibrosismedicineinatime-dependentmanner,especiallyinearlyrenalfibrosisstage.Targetedmetaboliteanalysisshowedthatthemedicinecouldlowerlevelsoflipid,acet
6、oacetate,glucose,phosphorylcholine/choline,trimethylamineoxideandraiselevelsofpyruvate,glycineintheserumoftherats.Serumclinicalchemistryandkidneyhistopathologyexaminationdovetailedwellwiththemetabonomicsdata.Conclusions/Significances:TheresultssubstantiatedthatCurcumaaromaticaoiladministrationc
7、anamelioraterenalfibrosissymptomsbyinhibitingsomemetabolicpathways,includinglipidsmetabolism,glycolysisandmethylaminemetabolism,whicharedominatingtargetsoftheagentworkinginvivo.Thisstudyfurtherstrengthensthenovelanalyticalapproach
