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页数:25页
时间:2019-07-11
《Reactor Engineering in Large Scale Animal Cell Culture 》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、Cytotechnology(2006)50:933DOI10.1007/s10616-006-9005-8ORIGINALPAPERReactorengineeringinlargescaleanimalcellcultureAlvinW.NienowReceived:29March2006/Accepted:29March2006/Publishedonline:Publishedonline:20June2006ÓSpringerScience+BusinessMediaB.V.2006AbstractThisarticlemainlyaddressestheissu
2、estionratethatimpactsonpHcontrol,pCO2andassociatedwiththeengineeringoflarge-scalefreeosmolality.Theseinteractionsaredifficulttore-suspensioncultureinagitatedbioreactorssolvebutifhigherspargeandagitationintensities>10,000Lbecausetheyhavebecomethesystemcouldbeusedtoachievethenecessaryoxygenof
3、choiceindustrially.Itisparticularlyconcernedtransfer,theproblemwouldlargelydisappear.withproblemsthatbecomeincreasinglyimpor-Thus,theperceptionofshearsensitivityisstilltantasthescaleincreases.However,veryfewimpactingonthedevelopmentofanimalcellpapershavebeenwrittenthatareactuallybasedcultu
4、reatthecommercialscale.Microcarrieronsuchlarge-scalestudiesandthefewthatdocultureisalsobrieflyaddressed.Finally,somerarelyaddressanyoftheissuesquantitatively.recommendationsforbioreactorconfigurationHence,itisnecessaryveryoftentoextrapolateandoperatingstrategyaregiven.fromsmall-scaleworkandt
5、hisreviewtriestopullthetwotypesofstudytogether.ItisshownthatKeywordsLarge-scalebioreactorsÆShearshearsensitivityduetoagitationandburstingsensitivityÆHomogeneityÆpHcontrolÆbubblesisnolongerconsideredamajorproblem.DissolvedCO2ÆOsmolalityHomogeneitybecomesincreasinglyimportantwithrespecttopHa
6、ndnutrientsatthelargestscaleandsub-surfacefeedingisrecommendedIntroductiondespitecleaninginplaceconcerns.Therearestillmajorquestionswithcellretention/recycleProbablythefirstdescriptionofanagitated,systemsatthesescales,eitherbecauseoffouling,stainlesssteelanimalcellculturebioreactorwasofcapa
7、cityorofpotentialanddifferentshearoneof30Lfortheproductionofinactivatedfootsensitivityquestions.Fed-batchoperationgivesandmouthdiseasevaccinefromfreelysuspendedrisetocelldensitiesthathaveledtotheuseofBHKcells(TellingandElsworth1965);andfromoxygenandenrichedair
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