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ID:39714032
大小:2.49 MB
页数:3页
时间:2019-07-09
《41 Neuroscience Application RevC Draft_No Tau data》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、ApplicationNoteNeuroscienceVisualizegeneexpressionandsplicejunctionsatthesingle-celllevelusingtheRNAscope®andBaseScope™insituhybridizationassaysSummaryInsitudetectionofG-proteinFigures1-4providebothfluorescentandcoupledreceptors(GPCRs)inthechromogenicimagesforfourtypesof
2、GPCRsThenervoussystemconsists-readilydetectedasmRNAtargetsbytheofnumerousspecializedcelladultmousebrainusingtheRNAscope®assay-inhippocampalandstriataltypesthatremaintobefullyRNAscope®ISHAssaycharacterizedatthemolecularnormalmousebrainareas:Dopaminergiclevel.Duetothehighd
3、egreeRaisingantibodiestoG-proteincoupledreceptorsReceptorsD1andD2(Drd1andDrd2),Cannabinoidofstructuralandfunctional(GPCRs)canbechallengingduetodifficultiesReceptor1(Cnr1),OpioidReceptorμ(OPRM1),δheterogeneityandtheintricateinobtainingsuitableantigenaccessibilitysince(OPR
4、D1),andκ(OPRK1),andCholinergicReceptorspatialorganizationoftheseGPCRsareoftenexpressedatlowlevelsandareMuscarinic3(Chrm3).cells,itisofspecialimportancetoveryunstablewhenpurified(Hutchingsetal.2010analyzegeneexpressionwithinthe(1)).Hereweshowexamplesforthedetectionof:morp
5、hologicalandspatialtissuecontext.TheRNAscope®insitu-DopaminergicReceptorshybridization(ISH)assayenables-CannabinoidReceptorhighlysensitiveandspecificgene-CholinergicReceptorexpressionvisualizationwithinthe-OpioidReceptorsnervoussystem.BaseScope™isaISHassaythatallowsvisua
6、lizationofsplicejunctionsbetweenadjacentexonsand/orretainedintronsinordertocharacterizealternativesplicingeventsincellsandtissues.ThisapplicationnotewillhighlightcasestudiesforbothISHassaysinthenervoussystem:•InsitudetectionofG-protein-FIGURE1.DetectionofDopamineReceptor
7、D1(Drd1,Red)andDopamineFIGURE 2.DetectionofCannabinoidreceptor1(Cnr1,Red)andcoupledreceptors(GPCRs)inReceptorD2(Drd2,Green)inmousebrainstriatumusingtheRNAscope®DopaminergicreceptorD1(Drd1,Green)innormalmousebrainstriatumtheadultmousebrainusingtheMultiplexFluorescentassay
8、onfreshfrozentissuesamples.DelineatedusingtheRNAscope®2.5HDDuplexChromogenicassayonFFPEtissueRNAscope®I
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