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1、ApplicationNoteNeuroscienceDetectionofRNAinthecentralandperipheralnervoussystemusingtheRNAscope®insituhybridizationassayAnneliesLaeremans,NaLi,MorganeRouault,YulingLuo,Xiao-JunMa,andEmilyParkAdvancedCellDiagnosticsInc.,7707GatewayBlvd,Newark,CA,USA94560
2、HighlightsIntroduction•Identification,characterization,NeuroscienceisoneofthefastestgrowingTherefore,thisrobustsignal-to-noisetechnologyand(co-)localizationofmRNAinterdisciplinaryresearchfieldsthatstudiestheallowsforthedetectionofgenetranscriptsatandlnc
3、RNAinthenervouscentral(CNS)andperipheralnervoussystemsinglemoleculelevelwithsingle-cellresolutionsystemfromthemolecularandcellularlevelstotheanalysisandcanfurtherexpandourunderstanding•Identification,visualizationandsystemslevel.Areasofresearchincludene
4、uralofgeneexpressionincelllinesandtissuescharacterizationofspecificcelldevelopment,structuralandfunctionalorganizationsamples.Themultiplexingcapabilitiesofboththetypesinthenervoussystemofthenervoussystem,cognitiveandbehavioralchromogenicandfluorescentRN
5、Ascope®assays•DetectionofmRNAintheneurosciences,andclinicalneurosciencesincludingfacilitatethesimultaneousvisualizationofmultiplenervoussystemwhennoneurodegenerativediseases.targetsinformalin-fixedparaffin-embedded(reliable)antibodiesare®(FFPE)andfresh/
6、fixedfrozensamples,enablingavailableTheRNAscopeinsituhybridization(ISH)assayconsistentcharacterizationofcellpopulationsprovidesapowerfulmethodtodetectgene•Visualizationofneuronalwithinthenervoussystem.Insummary,theexpressionwithinthespatialandmorphologi
7、calnetworkactivityandplasticityRNAscope®technologyallowsthevisualizationtissuecontext.Theproprietary“doubleZ”probe•ValidationoftargetmRNAandquantificationofvirtuallyanygenefromdesignincombinationwiththeadvancedsignalexpressionafterhigh-anygenomeinanytis
8、suewithunprecedentedamplificationenablesahighlyspecificandthroughputgeneexpressionspecificityandsensitivity.sensitivedetectionofthetargetRNAwitheachdotanalysisvisualizingasingleRNAtranscript.•Validationof(celltype-specificandcond
