免疫检测探针标记免疫金与银染增强的纳米粒子通过表面增强拉曼散射

免疫检测探针标记免疫金与银染增强的纳米粒子通过表面增强拉曼散射

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时间:2017-11-25

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1、Immunoassayusingprobe-labellingimmunogoldnanoparticleswithsilverstainingenhancementviasurface-enhancedRamanscatteringShupingXu,aXiaohuiJi,bWeiqingXu,*aXiaolingLi,aLianyingWang,bYubaiBai,*bBingZhaoaandYukihiroOzakicaKeyLaboratoryforSupramolecularStructureandMaterialofMinistryofEduc

2、ation,JilinUniversity,Changchun130021,P.R.ChinabCollegeofChemistry,JilinUniversity,Changchun130021,P.R.China.E-mail:wqxu@jlu.edu.cn;Fax:+86-431-8980729;Tel:+86-431-8498912cDepartmentofChemistry,SchoolofScienceandTechnology,Kwansei-GakuinUniversity,Sanda,Hyogo669-1337,JapanReceived

3、17thOctober2003,Accepted1stDecember2003FirstpublishedasanAdvanceArticleontheweb11thDecember2003Thispaperreportsanovelimmunoassaybasedonsurface-enhancedRamanscattering(SERS)andimmunogoldlabellingwithsilverstainingenhancement.ImmunoreactionsbetweenimmunogoldcolloidsmodifiedbyaRaman-

4、activeprobemolecule(e.g.,4-mercaptobenzoicacid)andantigens,whichwerecapturedbyantibody-assembledchipssuchassiliconorquartz,weredetectedviaSERSsignalsofRaman-activeprobemolecule.Alltheself-assembledstepsweresubjectedtothemeasurementsofultraviolet-visible(UV-vis)spectratomonitorthef

5、ormationofasandwichstructureontoasubstrate.Theimmunoassaywasperformedbyasandwichstructureconsistingofthreelayers.ThefirstlayerwascomposedofimmobilizedantibodymoleculesofmousepolyclonalantibodyagainstHepatitisBvirussurfaceantigen(PAb)onasiliconorquartzsubstrate.Thesecondlayerwasthe

6、complementaryHepatitisBvirussurfaceantigen(Antigen)moleculescapturedbyPAbonthesubstrate.Thethirdlayerwascomposedoftheprobe-labellingimmunogoldnanoparticles,whichweremodifiedbymousemonoclonalantibodyagainstHepatitisBvirussurfaceantigen(MAb)and4-mercaptobenzoicacid(MBA)astheRaman-ac

7、tiveprobeonthesurfaceofgoldcolloids.Aftersilverstainingenhancement,theantigenisidentifiedbyaSERSspectrumofMBA.AworkingcurveoftheintensityofaSERSsignalat1585cm21duetothen8aaromaticringvibrationofMBAversustheconcentrationofanalyte(Antigen)wasobtainedandthenon-optimizeddetectionlimit

8、fortheHepatitisBvirussurfaceantig

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