免疫检测的表面增强拉曼散射的基础上聚集的记者标记免疫纳米颗粒

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1、AnalBioanalChem(2008)392:187–193DOI10.1007/s00216-008-2237-zORIGINALPAPERImmunoassayusingsurface-enhancedRamanscatteringbasedonaggregationofreporter-labeledimmunogoldnanoparticlesJi-WeiChen&YongLei&Xiang-JiangLiu&Jian-HuiJiang&Guo-LiShen&Ru-QinYuReceived:7May2008/Revised:30May2008/Accepted:6

2、June2008/Publishedonline:4July2008#Springer-Verlag2008AbstractAone-stephomogenoussensitiveimmunoassayeffortsdevotedtothedevelopmentandtheapplicationofusingsurface-enhancedRamanscattering(SERS)hasbeenthistechnique[1–9].Generally,antigensweredetermineddeveloped.Thisstrategyisbasedontheaggregat

3、ionofbytheaggregationresultingfromthereactionoftheRamanreporter-labeledimmunogoldnanoparticlesinducedantibody-coatedparticleswiththecorrespondingantigens.bytheimmunoreactionwithcorrespondingantigens.TheRecentlyattentionhasturnedtowardstheapplicationofaggregationofgoldnanoparticlesresultsinaS

4、ERSsignalgoldnanoparticlesinaggregation-basedimmunoassaysincreaseoftheRamanreporter.Therefore,humanIgGowingtotheexcellentphysicalandchemicalcharacteristicscouldbedirectlydeterminedbymeasuringtheRamanofthesenanoparticles.Theproteinsboundtocolloidalgoldsignalofthereporter.Theprocessofaggregati

5、onwasparticlesareknowntobetterretainbiologicalactivityintheinvestigatedbytransmissionelectronmicroscopy(TEM)immunoassay[6,7].Therefore,severaladvantagescanbeandUV–Visabsorptionspectroscopy.Theeffectsoftheobtainedusinggoldnanoparticlesinaggregation-basedtemperature,time,andsizeofgoldnanoparti

6、clesontheimmunoassayssuchassimplepreparation,easyreadout,sensitivityoftheassaywereexamined.UsinghumanIgGandgoodstability.Apioneeringstudyontheapplicationofasamodelprotein,awidelineardynamicrange(0.1–goldnanoparticlesinaggregation-basedimmunoassayswas−115μgmL)wasreachedwithlowdetectionlimitpu

7、blishedbyThanhandRosenzweig[8].Intheirwork,−1(0.1μgmL)underoptimizedassayconditions.ThegoldnanoparticlescoatedwithproteinAwereusedtosuccessfultestsuggeststhattheapplicationoftheproposeddetermineanti-proteinAinaqueousandserumsolutions.methodholdspro