肺癌特异性靶向多肽的筛选及其在癌症诊疗方面的研究

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1、博士学位论文肺癌特异性靶向多肽的筛选及其在癌症诊疗方面的研究作者姓名：董兵指导教师:朱毅敏研究员中科院苏州纳米所阎锡蕴研究员中科院生物物理所学位类别:理学博士学科专业:生物物理学培养单位:中国科学院苏州纳米技术与纳米仿生研究所中国科学院生物物理研究所二零一二年五月ScreeningofspecifictargetingpeptideforlungcanercellsandapplicationofpeptidesincancerdiagnosisandtherapyByBingDongADissertationSubmitte

2、dtoGraduateUniversityofChineseAcademyofSciencesInpartialfulfillmentoftherequirementForthedegreeofDoctorofBiophysicsSuzhouInstitudeofNano-TechandNano-Bionics,ChineseAcademyofSciencesMay,2012关于学位论文使用权声明任何收存和保管本论文各种版本的单位和个人，未经著作权人授权，不得将本论文转借他人并复印、抄录、拍照、或以任何方式传播。否则，引起有碍

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4、规定）签名：导师签名：日期：…………………………………………………………………………………………关于学位论文原创性声明本人郑重声明：所呈交的学位论文是本人在导师指导下，独立进行研究工作所取得的成果。尽我所知，除文中已经注明引用的内容外，本学位论文的研究成果不包含任何他人享有著作权的内容。对本论文所涉及的研究工作做出贡献的其他个人和集体，均已在文中以明确方式标明。签名：导师签名：日期目录中文摘要·························································ⅠAbstract··

5、························································Ⅲ第一章绪论1.1肺癌的诊疗现状···········································11.2癌症分子靶向治疗的研究进展·······························31.3肺癌的生物标记物研究····································131.4靶向分子研究现状········································

6、181.5细菌展示技术进展及应用··································201.6药物载体进展及纳米技术的影响····························251.7课题设计思路及研究目标··································29第二章实验部分2.1实验材料················································312.2实验设备··············································

7、··312.3溶液配制················································322.4实验方法2.4.1共沉淀法筛选特异性细菌的方法·······················332.4.2流式细胞仪筛选特异性细菌的方法及流程···············342.4.3单克隆细菌分离方法·································362.4.4单克隆细菌结合效率比较实验·························372.4.5单克隆细菌结合效率及特异性表征

8、·····················382.4.6乙醇法固定细胞及细菌·······························402.4.7多聚甲醛法固定细胞细菌·····························412.4.8固定细菌的结合效率及特异性表征